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Home / Products / Primary Cells / Digestive System / Human Hepatic Stellate Cells

Cat. No. ARP0069

Human Hepatic Stellate Cells

Product Type:
Primary Cells
Species:
Homo sapiens (Human)
Tissue Source:
Liver

Human Hepatic Stellate Cell Medium

Datasheet

Hepatic Stellate Cells, also known as Ito cells, lipocytes, vitamin A-storing cells, fat-storing cells, exist in the space of Disse in the liver, between hepatocytes and sinusoidal endothelial cells. These cells functions in two states, quiescent and activated, and involve in vitamin A storage, liver regeneration, and response to liver injury. In a quiescent state, hepatic stellate cells store vitamin A (in the form of retinoids) in lipid droplets within their cytoplasm. They can be activated by liver injury, inflammation, metabolic stress, or toxin conditions. Upon activation, hepatic stellate cells secrete extracellular matrix (ECM), such as collagen type I and III, which initially helps protect the liver and maintain structural integrity, release growth factors that participate in liver regeneration, and produce cytokines, contributing to the immune response in the liver. Otherwise, α-SMA is a marker of hepatic stellate cell activation. It allows activated cells to contract, helping regulate sinusoidal blood flow and help compress damaged areas of the liver, and mediate cytoskeletal changes that facilitate cells migration to sites of liver injury. However, persistent activation of HSCs leads to excessive ECM deposition, driving liver fibrosis.

Human Hepatic Stellate Cells are freshly isolated from human health liver, cryopreserved at passage one and delivered frozen. Optimized medium and reagents for the growth of Human Hepatic Stellate Cells are also provided(Cat. No. ACM0069).

$1,900.00
/ Per Item

0.5 million

$1,900.00

In stock

Ships next business day

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Cell Type

Hepatic stellate cell
Culture Conditions

Growth medium

Human Hepatic Stellate Cell Medium (ARM0069)

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

Daily monitoring confirms that the cells are free from bacterial, yeast, and fungal contamination.

Mycoplasma testing

Negative for mycoplasma through PCR analysis

Pathogens

Cells tested negative for HIV-1, HBV, and HCV.
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

Reference
[1] L. Atzori, G. Poli, and A. Perra, “Hepatic stellate cell: A star cell in the liver,” Int. J. Biochem. Cell Biol., vol. 41, no. 8–9, pp. 1639–1642, Aug. 2009, doi: 10.1016/j.biocel.2009.03.001.
[2] A. E. Barry et al., “Hepatic Stellate Cells and Hepatocarcinogenesis,” Front. Cell Dev. Biol., vol. 8, p. 709, Aug. 2020, doi: 10.3389/fcell.2020.00709.
[3] H. Senoo, “Structure and function of hepatic stellate cells,” Med. Electron Microsc., vol. 37, no. 1, pp. 3–15, Mar. 2004, doi: 10.1007/s00795-003-0230-3.
[4] M. L. Hautekeete and A. Geerts, “The hepatic stellate (Ito) cell: its role in human liver disease,” Virchows Arch. Int. J. Pathol., vol. 430, no. 3, pp. 195–207, Mar. 1997, doi: 10.1007/BF01324802.
[5] B. J. Potter, “Components of the Hepatic System,” in Reference Module in Biomedical Sciences, Elsevier, 2019, p. B9780128012383049357. doi: 10.1016/B978-0-12-801238-3.04935-7.