Quick Order Cart
Promotion Products Primary Cells
Immortalized Cells Cancer (Tumor) Cell Lines
Genome-edited Cells Media and Reagents Organoids Uncategorized
Case Study

Case studies contain reports on ASCENT's exploration of the underlying mechanisms of cell states, including cell growth, cell differentiation, and even cell death.
Experiment Applications Preclinical Applications
Area of Research Interest
Industry
Case Study

Case studies contain reports on ASCENT's exploration of the underlying mechanisms of cell states, including cell growth, cell differentiation, and even cell death.
Applications Articles
Brochures and Flyers Protocols and Tips
Case Study
Our Product in Research Ascent Progress
Case Study

Case studies contain reports on ASCENT's exploration of the underlying mechanisms of cell states, including cell growth, cell differentiation, and even cell death.
Home / Service Details

lam Virtutem Ex Consuetudine Vitae Sermonisque

Hac ex causa conlaticia stipe Valerius humatur ille Publicola et subsidiis amicorum mariti inops cum liberis uxor alitur Reguli et dotatur ex aerario filia Scipionis...

Cell Immortralization Service

Cell immortalization offers a significant solution for extending the proliferative capacity of primary cells in culture, enabling long-term and reproducible research applications. Primary cells are directly isolated from fresh tissue and closely mimic in vivo conditions, making them ideal for various experiments. However, they eventually stop dividing, called replicative senescence. This biological limitation restricts their use for a long-term research project. Researchers often need to harvest fresh cells repeatedly, leading to batch-to-batch variability and reduced experimental reproducibility. Immortalization addresses these issues by modifying critical cellular mechanisms of proliferation, such as altering telomere regulation or suppressing senescence signaling pathways, allowing cells to bypass growth arrest and divide indefinitely. The following are several tools for immortalizing cells in culture conditions.

Shop Products
Telomerase Reverse Transcriptase protein (TERT) expression

Introducing TERT (telomerase reverse transcriptase) is a common method for immortalizing human cells that alters telomere regulation. Telomerase is a ribonucleoprotein enzyme that extends the DNA sequence of telomeres at the ends of chromosomes. By maintaining telomere length, telomerase enables cells to bypass replicative senescence and continue dividing indefinitely. While telomerase is typically inactive in most somatic cells, it is highly active in tumor cells. Taking advantage of this mechanism, TERT overexpression is widely used to achieve stable cell immortalization in research and biotechnology applications.

Tumor Suppressor Gene Inactivation

Another approach to cell immortalization involves the inactivation of tumor suppressor genes, such as p53 and Rb. These genes normally function to prevent uncontrolled cell proliferation. When they are repressed or inactivated, cells can bypass senescence and continue dividing.

One common method to achieve this is by introducing viral oncogenes, which are derived from viruses known to induce cancer. These oncogenes can inactivate tumor suppressor proteins and promote continuous cell growth. The most widely used viral oncogene is SV40 large T antigen, which inactivates both p53 and Rb. Other examples include:

EBV genes (EBNA1/EBNA2) - commonly used for B cell immortalization
HPV16 E6/E7 - effective in immortalizing keratinocytes
E1A gene from human adenovirus type 5 - used for epithelial cell immortalization, e.g. HEK293 is a human embryonic kidney cell immortalized by E1A of adenovirus 5.
c-Myc - a cellular oncogene that can drive proliferation and extend lifespan in some cell types.

Additionally, siRNAs targeting p53 or Rb can also be employed to transiently suppress these genes and support immortalization.

Strategy and Workflow

Cell immortalization service follows a standard and effective process to generate immortalized cell lines. The process starts with isolating primary cells from tissue samples, carefully preserving their original characteristics. Once isolated, we confirm their identity and the purity of the cells using immunostaining with a specific surface marker. Then, an immortalizing gene (e.g. SV40 T antigen) is introduced into the isolated cells with a resistance gene using lentiviral vectors. Antibiotic selection is then applied to enrich for successfully transduced cells. The surviving cells are allowed to grow and expand, forming an immortalized cell line. Immunostaining is finally performed again to check that the cells still show the expected markers and characteristics. This step-by-step process helps us create high-quality immortalized cell lines that are ready for your research or other applications.

How to Order

Please contact us with a brief description of your project or specific need (the cells you want to immortalize and the method you may be interested in). One of our experts will get in touch with you soon to discuss your requirement and support you with a customized strategy for free.

Reset Password

    Reach Us Questions? Click Me Here!

    Fill out the form below and a member of our team will contact you shortly!

    *Required field
    Referee Details Discounts will be issued to both accounts after manual review!

      🎁 Refer & Earn · Rewards for You & Your Friends

      ✨ Your Referral Link

      • Quality-Certified

        PCR assay ensures contamination-free research

      • Fast-Delivered

        Swift and dependable shipping to get your orders on time

      • Budget-Friendly

        Affordable solutions without compromising quality

      • Expert-Backed

        Professional 24/7 support from experienced specialists