ABCA2 Knockout Huh-7 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Huh-7 hepatocellular carcinoma cell line, featuring genetic disruption of the ABCA2 gene. This polyclonal population preserves the heterogeneous background of the parental line while enabling functional investigation of ABCA2 deficiency. The product is supplied as a ready-to-use polyclonal stock, suitable for expansion and downstream analyses, providing a robust loss-of-function model for ABCA2 biology.
The host Huh-7 cell line, established from a liver tumor of a 57-year-old Japanese male in 1982, is a well-differentiated hepatocellular carcinoma model widely used for studying hepatic function, hepatitis C virus replication, drug metabolism, and lipid homeostasis. These liver epithelial cells retain many hepatocyte characteristics, including metabolic enzyme expression and lipoprotein secretion, making them an appropriate system for investigating ABCA2-mediated lipid trafficking and drug resistance in a hepatocellular context.
ABCA2 encodes an intracellular ABC transporter that regulates subcellular cholesterol and sphingolipid distribution. It is transcriptionally controlled by SREBP-1c and LXR??, with modulation by PPAR?? and cellular cholesterol depletion. ABCA2 interacts with cholesterol, phospholipids, and the APP processing complex, influencing BACE1 and LRP1 activity, thus modulating lipid raft composition, sphingomyelin levels, and amyloid-beta peptide generation. In hepatocytes, ABCA2 contributes to hepatic lipid handling and drug resistance.
ABCA2 knockout in Huh-7 cells offers a platform to dissect hepatic lipid metabolism and drug resistance. Disruption of ABCA2 is expected to alter intracellular cholesterol and sphingolipid trafficking, affecting lipid droplet formation, membrane microdomains, and lipoprotein secretion. Additionally, ABCA2 has been implicated in multidrug resistance, and its loss enables evaluation of chemosensitivity. This model allows study of how ABCA2-mediated lipid redistribution influences tumor cell proliferation and apoptosis in hepatocellular carcinoma.
This polyclonal knockout product supports cholesterol efflux assays with filipin staining, LC-MS?Cbased lipidomics, sphingomyelin quantification, and drug sensitivity testing via MTT. Applications also include Western blotting, RT-qPCR, flow cytometry for lipid raft markers, immunofluorescence for lipid localization, and RNA-seq for transcriptomic profiling. These assays facilitate investigations into ABCA2-dependent lipid transport, regulatory networks, and contributions to Alzheimer??s disease pathways and cancer biology. For further information, contact Ascent Research.
