The ABCB10 Knockout MCF-7 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the MCF-7 human breast adenocarcinoma cell line. This product provides a loss-of-function model for studying the mitochondrial transporter ABCB10. The polyclonal format ensures a heterogeneous mixture of edited alleles, allowing robust assessment of ABCB10 disruption without clonal selection. This tool is suitable for investigating ABCB10-dependent pathways in breast cancer research.
The host cell line MCF-7 is an estrogen receptor-positive (ER+), progesterone receptor-positive (PR+), and human epidermal growth factor receptor 2-negative (HER2-) luminal A breast cancer model. Widely used in oncology research, MCF-7 cells retain key characteristics of hormone-responsive breast tumors, making them ideal for exploring metabolic and stress-response mechanisms relevant to cancer cell survival and proliferation.
ABCB10 encodes a mitochondrial inner membrane transporter essential for heme biosynthesis and mitochondrial iron homeostasis. It functions as an exporter of heme or heme precursors, directly interacting with ferrochelatase (FECH), ABCB7, and mitoferrin. Upstream regulators include transcription factors GATA1 and MITF, as well as oxidative stress signals. ABCB10 activity promotes the production of heme and iron-sulfur cluster proteins, while its loss leads to accumulation of oxidative stress and activation of apoptosis regulators. Within the heme biosynthesis pathway, ABCB10 operates alongside ALAS2 and FECH, and its function is coordinated with NRF2-mediated antioxidant responses.
Knockout of ABCB10 in MCF-7 cells disrupts mitochondrial function, impairing heme synthesis and reducing protection against oxidative stress. This sensitizes the cells to apoptosis, providing a powerful model for dissecting the interplay between mitochondrial metabolism and cell death pathways in luminal A breast cancer. The polyclonal knockout population avoids artifacts associated with clonal selection, enhancing the translational relevance of experimental findings.
These cells are suited for a range of applications including heme quantification assays, mitochondrial membrane potential measurements, ROS detection, and Seahorse metabolic profiling. They enable investigation of ABCB10 in iron metabolism, oxidative stress resistance, and cancer cell metabolism. Standard assays such as western blotting, RT-qPCR, and flow cytometry can confirm ABCB10 disruption and downstream effects on apoptosis. Researchers can employ these cells to evaluate therapeutic strategies targeting mitochondrial vulnerabilities in breast cancer. For further information or to request a quote, please contact Ascent Research.
