The ABCB10 Knockout T-47D Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human breast cancer cell line T-47D, offering a loss-of-function model for the ABCB10 gene. This polyclonal pool contains diverse edited alleles, providing a robust tool for initial functional screening without clonal biases. The gene disruption is achieved via CRISPR/Cas9, enabling researchers to study ABCB10 deficiency in a disease-relevant context.
T-47D is a widely used luminal B breast cancer cell line established from a pleural effusion of a ductal carcinoma patient. These epithelial cells are estrogen receptor (ER)-, progesterone receptor (PR)-, and HER2-positive, and harbor PIK3CA and TP53 mutations, reflecting key features of hormone-responsive breast cancer. The line??s well-characterized molecular landscape makes it suitable for studying gene function in breast cancer biology.
ABCB10 encodes a mitochondrial inner membrane transporter essential for heme biosynthesis and iron homeostasis, and for protection against oxidative stress. Transcription is regulated by GATA1, TP53, and NFE2L2. ABCB10 interacts with ferrochelatase (FECH) and ALAS2 to facilitate heme precursor transport, and functionally relates to ABCB7. Downstream, it modulates expression of HMOX1, SOD2, and FECH, linking mitochondrial transport to antioxidant defenses.
In T-47D cells, ABCB10 disruption likely impairs mitochondrial iron homeostasis and increases oxidative stress susceptibility, potentially affecting tumor cell viability. Since these cells express ER, PR, and HER2, the model enables study of crosstalk between heme metabolism and hormone signaling, and may alter responses to therapies like tamoxifen. This model aids in dissecting the role of mitochondrial transporters in cancer progression and drug resistance.
Applications include investigation of mitochondrial heme transport using heme quantification, mitochondrial iron measurement, and ROS detection assays. The knockout cells support western blotting and RT-qPCR validation of targets (e.g., HMOX1, SOD2) and regulators (e.g., GATA1, TP53), and functional assays such as cell viability, apoptosis, colony formation, and migration. They are suitable for drug screening to target iron addiction or oxidative stress vulnerabilities, and for evaluating ABCB10 as a therapeutic target. For further information, please contact Ascent Research.
