The ABCF3 Knockout Huh-7 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Huh-7 human hepatocellular carcinoma cell line. This loss-of-function model targets the ABCF3 gene via CRISPR/Cas9-mediated gene disruption, generating a heterogeneous pool of edited cells. The polyclonal format preserves genetic diversity, enabling robust analysis of ABCF3-dependent phenotypes in a liver cancer context. No clonal selection has been applied, ensuring representation of multiple knockout variants.
Huh-7 is a well-differentiated hepatocellular carcinoma cell line extensively used as a model for hepatocyte biology, drug metabolism, and liver cancer research. These cells retain key hepatic functions, including albumin secretion and metabolic enzyme activity, and serve as a platform for studying hepatocellular carcinoma pathogenesis and therapeutic responses. The ABCF3 knockout in this background facilitates dissection of gene function in liver cancer cell proliferation, drug sensitivity, and translation control.
ABCF3 is a non-transporter ABC protein that functions as a positive regulator of translation and ribosome biogenesis. It operates downstream of the MYC oncogene and interacts with ribosomal proteins and translation initiation factors to promote global protein synthesis. ABCF3 activity converges on the mTOR signaling pathway, influencing downstream kinases eIF4E and S6K to drive cap-dependent translation and cell proliferation. Disruption of ABCF3 attenuates mTOR-mediated translation, impairing protein synthesis and cell cycle progression.
In the Huh-7 hepatocellular carcinoma model, ABCF3 knockout impairs protein synthesis and cell proliferation, underscoring its role in maintaining the transformed state. Reduced ribosome biogenesis and translation rates diminish tumorigenic potential, making these polyclonal cells a valuable system for studying hepatocellular carcinoma biology. This model allows investigation of ABCF3 as a therapeutic target and its involvement in drug sensitivity, particularly to agents affecting mTOR signaling or translation. The heterogeneous knockout pool mimics tumor heterogeneity, providing translational relevance.
Applications include functional analysis of ABCF3 in translation regulation, cancer cell biology, and drug sensitivity screening. Representative techniques encompass western blotting, RT-qPCR, cell proliferation assays, polysome profiling, and RNA-seq. Researchers can employ this model to explore ABCF3-dependent signaling networks, screen for modulators of translation, or study mTOR?Cribosome biogenesis crosstalk in liver cancer. For additional information or ordering, contact Ascent Research.
