The ABCF3 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Jurkat human T lymphocyte cell line. This product features targeted disruption of the ABCF3 gene, encoding an interferon-inducible ATP-binding cassette protein, in a heterogeneous pool of cells. The polyclonal format provides a robust loss-of-function model for studying ABCF3-dependent processes without clonal selection. Supplied as a ready-to-use suspension culture suitable for downstream functional assays.
The Jurkat parental line is an immortalized CD4+ T cell line from a patient with acute T cell leukemia. Expressing the T cell receptor, these cells are responsive to T cell activation stimuli and serve as a model for T cell signaling and apoptosis. Jurkat cells are widely used for studying HIV infection, interferon responses, and T cell biology due to well-characterized signaling properties.
ABCF3 is an interferon-inducible translation inhibitor upregulated via the JAK-STAT pathway. Interferon-alpha/beta binding to IFNAR1/IFNAR2 activates JAK1 and TYK2, phosphorylating STAT1 and STAT2. Phosphorylated STAT1-STAT2 heterodimers recruit IRF9 to form ISGF3, which drives ABCF3 transcription. ABCF3 then binds eIF2 alpha/beta on ribosomes, stalling translation initiation and restricting viral replication while modulating host protein synthesis. This mechanism contributes to antiviral innate immunity and intersects with PKR and GCN2 pathways.
In Jurkat T cells, ABCF3 knockout enables dissection of interferon signaling and translational control. With intact JAK-STAT machinery, these cells allow direct assessment of ABCF3??s role in T cell responses to interferons or viral challenges. The model helps separate ABCF3-mediated translation regulation from other interferon-stimulated gene functions. Additionally, the leukemic background makes it valuable for exploring ABCF3 in T cell malignancies and autoimmune disorders where interferon signaling is perturbed.
Key applications include antiviral innate immunity studies, translational control analysis, and screening for interferon pathway modulators. Typical assays include RT-qPCR for ABCF3 expression, western blotting for STAT1 phosphorylation, interferon stimulation assays, polysome profiling, viral replication assays, and flow cytometry for T cell activation. These cells provide a platform for mechanistic and drug discovery research. For further information, contact Ascent Research.
