ABI2 Knockout HAP1 Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Bone Marrow
Disease:
Chronic myeloid leukemia

Datasheet

CRISPR/Cas9-edited polyclonal knockout cells targeting ABI2 in the near-haploid HAP1 cell line. This loss-of-function model enables dissection of ABI2-mediated linkage between Rac1 and Abl kinase signaling and the WAVE regulatory complex during actin polymerization, lamellipodia formation, and cell migration. Ideal for studying actin dynamics, invasion, and kinase crosstalk in a BCR-ABL1-positive leukemic background. Suitable for western blotting, immunofluorescence, scratch wound migration, transwell invasion, co-immunoprecipitation, and phospho-signaling assays.

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Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

HAP1

Sex of Donor

Male

Age

40 years

Derived From Site

Bone marrow

Gene Name

ABI2

Gene Identifier

NCBI Gene ID 10152

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

IMDM

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

ABI2 Knockout HAP1 Polyclonal Cells comprise a CRISPR/Cas9-edited polyclonal knockout cell population engineered for targeted disruption of the ABI2 gene in the HAP1 cell line.

This product provides a loss-of-function model system to investigate ABI2-dependent cellular processes.

The polyclonal format encompasses a pool of edited cells with heterogeneous gene-disruption events, enabling robust population-level functional studies without single-cell cloning.

Researchers can employ this model to interrogate ABI2??s role in actin cytoskeleton dynamics and signal transduction pathways.