The ACBD3 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population for loss-of-function studies of the ACBD3 gene in a human T-cell line. This heterogeneous pool of Jurkat cells carries targeted gene disruption, enabling investigation of ACBD3 deficiency without clonal selection. The polyclonal format maintains population diversity, suitable for experiments not requiring clonal uniformity while reliably probing ACBD3-dependent pathways.
The parental Jurkat cell line is an immortalized T-lymphocyte line from a T-cell leukemia patient, widely used to study T-cell receptor signaling, apoptosis, and HIV infection. Its robust growth and well-mapped signaling networks make it ideal for examining immune cell functions and secretory pathways. Employing Jurkat for ACBD3 knockout permits exploration of Golgi biology in the context of T-cell physiology.
ACBD3 (GCP60) is a Golgi-associated acyl-CoA binding protein that interacts with giantin (GOLGB1) to maintain Golgi stacking and functions as a co-chaperone for HSP90AA1. It promotes maturation of clients like CYP11A1 and LDL receptor, and is transcriptionally regulated by SREBP2 in response to cholesterol. Downstream, ACBD3 governs Golgi morphology, secretory transport, and lipid metabolism. It also binds viral proteins (enterovirus 3A, HCV NS5A), implicating it in replication. Knockout of ACBD3 causes Golgi fragmentation, impaired secretion, and lipid dysregulation, highlighting its scaffolding role at the Golgi?CHSP90 interface.
In Jurkat T cells, ACBD3 knockout disrupts Golgi integrity, potentially impairing cytokine secretion, surface receptor presentation, and apoptotic signaling. This model is valuable for dissecting Golgi-dependent steps in T-cell activation and for studying host factors in enterovirus/HCV replication, as these viruses exploit ACBD3. Additionally, it can inform on ACBD3??s role in leukemogenesis and neurodevelopment due to its involvement in HSP90 chaperone cycles and steroidogenesis.
Applications include Western blotting and immunofluorescence for ACBD3 and giantin to confirm knockout and visualize Golgi fragmentation. Flow cytometry can assess surface LDL receptor levels and Annexin V apoptosis, while co-immunoprecipitation probes HSP90 interactions. Cytokine ELISAs and RT-qPCR measure functional secretion and transcriptional changes. Viral replication assays extend utility to virology. These polyclonal knockout cells provide a versatile tool for cell biology, immunology, and drug discovery. For further details, contact Ascent Research.
