ACE2 Knockout KYSE-150 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population enabling loss-of-function investigation of the ACE2 gene in a human esophageal squamous cell carcinoma model. The heterogeneous pool of gene-disrupted cells provides a powerful system for studying ACE2-dependent signaling, viral entry mechanisms, and tumor biology without the clonal artifacts associated with monoclonal lines.
The parental KYSE-150 cell line originates from a poorly differentiated esophageal squamous cell carcinoma resected from a 49-year-old Japanese female. This line is extensively characterized for esophageal cancer studies, offering a clinically relevant context for examining ACE2 function in carcinogenesis and host?Cpathogen interactions.
ACE2 encodes a transmembrane carboxypeptidase that converts the vasoconstrictor angiotensin II to the vasodilatory angiotensin 1?C7, which activates the Mas receptor to counteract the renin?Cangiotensin system. ACE2 also serves as the cellular receptor for SARS-CoV and SARS-CoV-2 spike proteins, requiring TMPRSS2-mediated priming and ADAM17 sheddase activity. The protein interacts with CALM and is regulated by angiotensin II, angiotensin 1?C7, and viral spike protein. Downstream, angiotensin 1?C7 signals through Mas and AT2 receptors, and ACE2 activity influences the bradykinin B1/B2 receptor and apelin pathways. In esophageal cancer, ACE2 may modulate proliferation and the tumor microenvironment.
Within the KYSE-150 esophageal cancer background, ACE2 knockout allows dissection of the enzyme’s impact on angiotensin/bradykinin-mediated signaling, cell growth, and susceptibility to viral infection. The model is pertinent for exploring how ACE2 loss alters cancer cell behavior and for evaluating the esophageal epithelium’s response to SARS-CoV-2 entry, given the reported ACE2 expression in esophageal tissues.
Key research applications encompass SARS-CoV-2 pseudovirus entry assays, angiotensin metabolism profiling through enzymatic activity measurements, and protein interaction analyses via co-immunoprecipitation with spike protein. Additional applications include drug screening for ACE2 modulators, migration/invasion studies, immunofluorescence, Western blotting, RT-qPCR, and flow cytometry for surface ACE2. These polyclonal knockout cells offer a versatile tool for both cancer biology and virology. For technical consultations, contact Ascent Research.
