The ACP1 Knockout HEK293T Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population with targeted disruption of the ACP1 gene. This heterogeneous pool minimizes clonal artifacts and preserves biological complexity for unbiased functional studies. Without single-cell cloning, the polyclonal format ensures robust representation of gene-edited cells, making it an ideal loss-of-function model for investigating ACP1-mediated signaling in a human cellular context.
HEK293T is a widely used human embryonic kidney cell line derived from HEK293 cells transformed with sheared adenovirus 5 DNA. It stably expresses the SV40 large T antigen, enabling episomal replication of plasmids containing the SV40 origin, thereby facilitating high-level transient protein expression. These features, combined with high transfection efficiency and rapid growth, make HEK293T a preferred host for signaling studies and functional genomics applications.
ACP1 encodes a low molecular weight phosphotyrosine phosphatase (LMW-PTP) that dephosphorylates phosphotyrosine-containing substrates, functioning as a negative regulator in growth factor and integrin signaling. Upstream signals from growth factors (PDGF, insulin, EGF), cytokines (IL-2), T cell receptor, and integrins activate ACP1, which then targets PDGFR, EphA2, STAT5, p190RhoGAP, FAK, and paxillin to attenuate downstream cascades. Interactions with Grb2, SHP-2, Csk, and caveolin-1 further modulate signal propagation. Consequently, ACP1 knockout results in sustained tyrosine phosphorylation, enhancing Src family kinase, RhoA, and Rac1 activity, and promoting proliferation and migration.
In HEK293T cells, ACP1 knockout provides a clean genetic background to dissect its role in phosphotyrosine-dependent pathways without endogenous phosphatase interference. The polyclonal population avoids clonal selection artifacts, offering more physiologically relevant signaling dynamics. The SV40 large T antigen supports episomal replication, enabling rescue experiments to validate ACP1 function. This model is particularly valuable for quantifying ligand-induced phosphorylation events and downstream effector activation in a human cell system amenable to high-throughput handling.
Applications include studying cancer cell migration, growth factor receptor regulation, immune signaling, and redox control of phosphatases. Common assays include Western blotting for phospho-tyrosine, immunofluorescence for focal adhesion markers (paxillin, FAK), transwell migration, co-immunoprecipitation of receptor complexes, and phosphatase activity measurements. The cells are also suited for drug sensitivity screening with kinase inhibitors and phospho-specific flow cytometry. For further details, contact Ascent Research.
