The ACTA1 Knockout A2780 Polyclonal Cells product provides a CRISPR/Cas9-edited polyclonal cell population derived from the A2780 human ovarian carcinoma cell line, designed for targeted disruption of the ACTA1 gene. This heterogeneous knockout pool enables loss-of-function studies without clonal selection artifacts, maintaining the genetic diversity inherent to polyclonal populations. It is a robust tool for investigating ACTA1-dependent mechanisms in ovarian cancer biology.
The A2780 parental line, established from an untreated patient with ovarian endometrioid adenocarcinoma, displays adherent epithelial morphology and is widely used to model ovarian cancer processes including proliferation, apoptosis, chemoresistance, and metastasis. Its well-documented background makes it a reliable platform for examining cytoskeletal contributions to tumor cell behavior.
ACTA1 encodes skeletal muscle ??-actin, which can integrate into the cytoskeleton of non-muscle cells, influencing actin polymerization and contractility. The protein operates downstream of Rho GTPase signaling (RhoA, ROCK, LIMK) and SRF/MRTF transcriptional regulation, and it directly interacts with actin-modulating factors such as tropomyosin, cofilin, and the Arp2/3 complex. ACTA1 activity promotes focal adhesion maturation, myosin II activation, and matrix metalloproteinase secretion, with key downstream mediators including FAK and Src kinases.
In A2780 ovarian cancer cells, ACTA1 knockout disrupts filamentous actin structures, impairing cell motility, adhesion, and invasive potential by destabilizing focal adhesions and reducing actomyosin contractility. This model is particularly relevant for studying aberrant cytoskeletal regulation in epithelial cancers and may provide insights into the non-muscle roles of ??-actin isoforms.
Researchers can apply these cells to migration and invasion assays (wound healing, Transwell), F-actin visualization with phalloidin, immunofluorescence for focal adhesion components, and Rho GTPase activity measurements. They are also valuable for drug screening targeting actin dynamics and for exploring chemoresistance mechanisms tied to cytoskeletal remodeling. To obtain this product or seek technical support, please contact Ascent Research.
