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Home / Products / Genome-edited Cells / Knockout Cell Lines / ADAL Knockout HT29 Polyclonal Cells
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Cat. No. ARG32853

ADAL Knockout HT29 Polyclonal Cells

  • Product Type:

    Polyclonal Cell Population

  • Species:

    Homo sapiens (Human)

MAPDA knockout HT29 polyclonal cells are a CRISPR/Cas9-edited loss-of-function model targeting the scaffold protein MAPDA in human colorectal adenocarcinoma cells. Disruption of MAPDA, which facilitates MAPK signal transduction through interactions with MEK1 and ERK2, alters phosphorylation of downstream effectors such as ELK1 and c-Jun, impairing proliferation and survival signaling. These polyclonal cells derive from the HT29 intestinal epithelial line, widely used for colorectal cancer and drug transport studies, and are optimized for applications including Western blotting, apoptosis assays, and targeted therapy screening in MAPK-driven disease research.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice


Disclaimer:

For Research Use Only

  • Characteristics

    Host Cell

    HT29

    Gene Name

    ADAL

    Gene Identifier

    NCBI Gene ID 161823

    Storage

    Liquid nitrogen (LN2)

  • Culture Conditions

    Growth medium

    McCoy's 5A

    Supplement(s)

    10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

    Temperature

    37°C

    Atmosphere

    5% COâ‚‚

  • Quality Control

    Sterility testing

    The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

    Mycoplasma testing

    Negative for mycoplasma through PCR analysis

  • Disclaimer

    Intended Use

    This product is intended for laboratory in vitro use only. It is not intended for diagnostic, therapeutic, or clinical applications.

    Disclaimer

    Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

    By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

    This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The MAPDA Knockout HT29 Polyclonal Cells product comprises a CRISPR/Cas9-edited polyclonal knockout population of the human colorectal adenocarcinoma cell line HT29, engineered to disrupt expression of the MAPDA gene.

This loss-of-function model provides researchers with a robust tool to interrogate the scaffold protein??s role in the mitogen-activated protein kinase (MAPK) signaling cascade.

By eliminating functional MAPDA, the cells enable dissection of kinase pathway architecture without the confounding presence of wild-type protein across a heterogeneous cell pool, reflecting the polyclonal nature of the knockout and avoiding artifacts associated with single-cell cloning.

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