The ADAMTS14 Knockout MCF-7 Polyclonal Cells comprise a population of MCF-7 cells engineered via CRISPR/Cas9-mediated gene disruption to eliminate functional ADAMTS14 expression. This polyclonal knockout product provides a loss-of-function model for investigating the role of the ADAMTS14 metalloproteinase in extracellular matrix remodeling and collagen homeostasis. The polyclonal format minimizes clonal biases and captures a diverse range of genetic alterations, enabling robust functional studies.
The parental MCF-7 cell line is an estrogen receptor-positive, progesterone receptor-positive, and HER2-negative human breast adenocarcinoma epithelial line isolated from a pleural effusion. It serves as a well-established model for luminal A breast cancer, retaining epithelial morphology and hormone responsiveness. MCF-7 cells are widely employed in cancer research to study cell adhesion, migration, and the molecular determinants of metastatic behavior, making them a suitable host for examining ADAMTS14-mediated matrix modulation.
ADAMTS14 is a secreted metalloproteinase that functions as a procollagen N-propeptidase, cleaving the N-propeptides of procollagen I and III to produce mature collagens required for fibril assembly. Its activity is regulated upstream by TGFB1 and IL1B, and its transcription is influenced by NFKB1 and SOX9. Within the extracellular matrix, ADAMTS14 interacts with procollagen substrates and fibronectin, and its proteolytic action governs the formation of collagen fibrils and overall matrix integrity. Consequently, ADAMTS14 knockout is predicted to disrupt collagen fibrillogenesis and downstream cell-matrix adhesion signaling.
In the MCF-7 breast cancer context, loss of ADAMTS14 impairs collagen maturation, leading to defective ECM organization that can alter cell adhesion, migration, and invasion??key processes in metastasis. This model enables dissection of how metalloproteinase-driven ECM remodeling influences the transition from an epithelial to a more motile phenotype. Furthermore, given ADAMTS14??s association with osteoarthritis and skeletal dysplasia, the knockout cells may also be relevant for studying matrix pathologies in joint and bone diseases.
Key applications include collagen processing studies, ECM dynamics in cancer, and breast cancer metastasis research. Representative assays such as Western blotting for ADAMTS14 and collagens, procollagen cleavage assays, cell invasion/migration Boyden chamber assays, and collagen fiber immunofluorescence allow direct evaluation of ADAMTS14 function. RT-qPCR for ECM genes and zymography for gelatinase activity further complement analysis of matrix remodeling. For technical inquiries and ordering, please contact Ascent Research.
