The ADAMTS14 Knockout SK-OV-3 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the SK-OV-3 human ovarian adenocarcinoma line. This loss-of-function model targets the ADAMTS14 gene, enabling investigation of its role in extracellular matrix biology and ovarian cancer. The polyclonal format provides a heterogeneous pool of edited cells, facilitating functional studies without clonal bias.
SK-OV-3 is an epithelial ovarian cancer cell line established from the ascites of a patient with ovarian adenocarcinoma. It is a widely used model for studying ovarian cancer biology, drug resistance, and metastasis, thanks to its well-characterized molecular profile and tumorigenic capacity in xenografts, making it ideal for examining cell-ECM interactions in disease-relevant contexts.
ADAMTS14 is a secreted metalloprotease with procollagen N-proteinase activity, essential for cleaving the N-terminal propeptides of procollagens I and II to facilitate collagen fibril assembly and ECM organization. Its function is regulated by TGF-??, mechanical stress, and IL-1??, and it directly interacts with substrates procollagen I/II, the inhibitor TIMP-3, and fibronectin. Downstream, it produces mature collagen I/II fibrils. Knockout of ADAMTS14 disrupts collagen processing, potentially impairing cell adhesion, migration, and TGF-?? pathway signaling, thereby altering the tumor microenvironment architecture.
In SK-OV-3 cells, ADAMTS14 knockout provides a model to investigate how defective collagen maturation impacts ovarian cancer progression. Ovarian tumors rely on ECM remodeling for invasion and metastasis; thus, loss of ADAMTS14 allows dissection of its role in collagen fibril formation, TGF-?? signaling, and matrix stiffness. This model is valuable for studying tumor-stroma crosstalk and the ECM??s contribution to chemoresistance in an ovarian adenocarcinoma context.
The knockout cells are suitable for assays including Western blotting to detect procollagen processing defects, migration/invasion assays using Boyden chambers, immunofluorescence for collagen and ECM markers, and RT-qPCR for ECM gene expression. Collagen gel contraction assays gauge functional matrix remodeling, co-immunoprecipitation probes ADAMTS14 interactions, and phospho-signaling analysis can profile TGF-?? pathway activity. These applications make the ADAMTS14 knockout cells a robust tool for exploring ovarian cancer ECM dynamics. For inquiries, contact Ascent Research.
