The AIF1 Knockout CAL-27 Polyclonal Cells are a heterogeneous population of CAL-27 oral squamous cell carcinoma cells with targeted disruption of the AIF1 gene achieved through CRISPR/Cas9-mediated genome editing. This product is supplied as a polyclonal knockout pool, representing a mixed population of edited cells, and provides a loss-of-function model for investigating AIF1 biology in a human cancer background.
CAL-27 is an established cell line derived from a human tongue squamous cell carcinoma, characterized by its adherent epithelial morphology. It serves as a widely utilized in vitro model for oral squamous cell carcinoma, enabling studies of tumor cell proliferation, invasion, and interactions within the tumor microenvironment.
AIF1 (allograft inflammatory factor 1) encodes a cytoplasmic calcium-binding protein that functions as a sensor for inflammatory stimuli. It is activated by upstream signals including IFN-??, TNF-??, LPS, IL-1??, and CSF-1, and in turn promotes downstream signaling through NF-??B (engaging NFKB1 and RELA) and MAPK (involving MAPK1 and MAPK3) pathways. AIF1 also interacts with F-actin, L-plastin, and PAK1 to orchestrate actin cytoskeleton remodeling, thereby modulating cell motility and regulating the secretion of cytokines such as IL-6, IL-10, TNF-??, and CCL2, as well as the activity of the ACTR2/3 complex.
Knockout of AIF1 in CAL-27 cells is expected to impair inflammatory signaling and actin-dependent processes, leading to reduced activation of NF-??B and MAPK cascades, diminished pro-inflammatory cytokine output, and attenuated migratory and invasive capacity. This engineered model thus enables precise dissection of AIF1??s contribution to the aggressive behavior of oral squamous cell carcinoma and its interplay with immune components in the tumor niche.
Researchers can employ this knockout cell population in a variety of functional assays, including wound healing and transwell migration experiments to assess motility, co-culture systems with macrophages to study immune-cancer crosstalk, and drug screening efforts aimed at AIF1-regulated pathways. Typical analytical techniques comprise western blotting, RT-qPCR, immunofluorescence, ELISA for cytokine quantification, phalloidin staining for F-actin visualization, and phospho-NF-??B p65 detection. For further details or technical inquiries, please contact Ascent Research.
