The AIMP1 Knockout HT29 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population engineered to disrupt the AIMP1 gene in HT29 human colorectal adenocarcinoma cells. This loss-of-function model maintains genetic heterogeneity, avoiding clonal artifacts, and provides a robust tool for studying AIMP1-dependent mechanisms. The polyclonal format is ideal for pooled functional assays, signaling studies, and rescue experiments, ensuring representative results from a diverse population of edited cells.
HT29 cells are a human colorectal adenocarcinoma epithelial line capable of enterocytic differentiation under appropriate conditions. Widely employed as a model for intestinal epithelial cell biology, drug absorption, and colorectal cancer, these cells retain intact MAPK, NF-kB, and hypoxia signaling pathways. This background is highly relevant for investigating AIMP1 functions in colorectal tumor biology, where inflammatory and angiogenic processes play critical roles.
AIMP1 is a scaffold protein within the multisynthetase complex, interacting with AIMP2, AIMP3, KARS, MARS, and HSP90 to stabilize aminoacyl-tRNA synthetases. Upon stimulation by TNF-alpha, IL-1beta, LPS, or hypoxia, AIMP1 is proteolytically released and secreted as a cytokine. The secreted form activates MAPK cascades (ERK1/2, JNK, p38) and NF-kB signaling, leading to transcriptional induction of IL-6, IL-8, MCP-1, MMP2, MMP9, and HIF-1alpha. Through these actions, AIMP1 couples translational control to inflammation, angiogenesis, and tissue remodeling.
In HT29 cells, AIMP1 knockout enables dissection of its role in intestinal epithelial inflammation and angiogenesis relevant to colorectal cancer. Loss of AIMP1 expression impairs stress-induced cytokine secretion and modulates responses of the MAPK and NF-kB networks. This model is particularly useful for investigating how AIMP1 contributes to tumor cell migration, invasion, and the angiogenic switch, as well as for testing modulators of the multisynthetase complex and downstream inflammatory mediators.
Typical applications include western blotting, RT-qPCR, cytokine ELISAs, phospho-protein analysis, cell proliferation, migration, and invasion assays, apoptosis detection, and tube formation experiments. The cells are also suitable for co-immunoprecipitation of multisynthetase complex components and NF-kB luciferase reporter assays. Researchers can utilize this knockout model to screen for regulators of AIMP1 secretion or to perform functional rescue studies. For additional technical information, please contact Ascent Research.
