ALDH1B1 Knockout CaSki Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Uterus (cervix)
Disease:
Squamous cell carcinoma

Datasheet

The ALDH1B1 Knockout Ca Ski Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Ca Ski cervical carcinoma line. This model disrupts ALDH1B1, encoding a mitochondrial aldehyde dehydrogenase that oxidizes acetaldehyde and retinaldehyde, supporting retinoic acid signaling and aldehyde detoxification. These cells are applicable to cancer stem cell biology, drug resistance, alcohol-related carcinogenesis, and retinoid signaling research. They facilitate Aldefluor flow cytometry, Western blotting, RT-qPCR, and functional assays for aldehyde clearance and retinoic acid quantification.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

CaSki

Sex of Donor

Female

Age

40 years

Derived From Site

Metastatic; Small intestine

Gene Name

ALDH1B1

Gene Identifier

NCBI Gene ID 219

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The ALDH1B1 Knockout Ca Ski Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from human cervical carcinoma epithelial Ca Ski cells.

This product carries a targeted disruption of the ALDH1B1 gene, generated via CRISPR/Cas9-mediated genome editing, creating a loss-of-function model for investigating mitochondrial aldehyde dehydrogenase 1 family member B1.

The polyclonal nature preserves genetic heterogeneity and provides a robust system for downstream functional analyses, enabling reproducible assessment of ALDH1B1-dependent phenotypes in aldehyde metabolism and retinoid synthesis.