The ALDH3A2 Knockout Jurkat Polyclonal Cells consist of a CRISPR/Cas9-edited polyclonal knockout cell population derived from Jurkat human T lymphocytes, in which the ALDH3A2 gene is disrupted. This loss-of-function model enables study of fatty aldehyde dehydrogenase deficiency in a readily expandable format suitable for pooled assays, avoiding the need for clonal isolation.
Jurkat cells are an immortalized human T-cell line originally isolated from the peripheral blood of a 14-year-old male with acute T-cell leukemia. They are extensively used for probing T-cell activation, signal transduction, and immune function, owing to their well-characterized signaling networks and amenability to genetic manipulation.
The ALDH3A2 gene product is an NAD+-dependent fatty aldehyde dehydrogenase that oxidizes long-chain aliphatic aldehydes derived from fatty alcohol metabolism and sphingolipid degradation, converting them to non-toxic fatty acids. The enzyme forms a functional homodimer and is transcriptionally controlled by NRF2 under oxidative stress, with further regulation by PPAR?? and PPAR??, placing it at the intersection of aldehyde detoxification and lipid metabolism. It acts downstream of sphingosine-1-phosphate breakdown, and its loss leads to accumulation of reactive aldehydes that promote cellular damage.
In Jurkat T cells, ALDH3A2 knockout models the consequences of impaired aldehyde clearance within a lymphocyte environment. T cells depend on lipid signaling for activation and survival, so disrupted aldehyde metabolism may alter immune responsiveness and stress tolerance. This model is particularly pertinent to Sj?gren-Larsson syndrome, a rare disorder caused by ALDH3A2 mutations, enabling investigation of how aldehyde toxicity influences T-cell physiology.
Applications include mechanistic studies of fatty aldehyde dehydrogenase deficiency, high-throughput screening for compounds that mitigate aldehyde toxicity, and examination of oxidative stress responses in immune cells. The polyclonal knockout cells are compatible with aldehyde dehydrogenase activity assays, mass spectrometry-based lipidomics, reactive aldehyde quantification, RT-qPCR, western blotting, and cell viability tests under oxidative challenge. For detailed product information and technical support, please contact Ascent Research.
