The ALMS1 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population providing a loss-of-function model for the ALMS1 gene in Jurkat T lymphocyte leukemia cells. ALMS1 encodes a centrosome-associated protein essential for primary cilia formation, intracellular trafficking, and cell cycle regulation. CRISPR/Cas9-mediated gene disruption generates a heterogeneous pool of ALMS1-knockout cells suitable for population-based functional studies.
The Jurkat cell line, derived from human acute T-cell leukemia, is an immortalized T-lymphocyte model widely used for T-cell receptor signaling, apoptosis, and cancer research. These cells express key T-cell markers and signaling pathways, and although they are non-ciliated in standard culture, ciliogenesis can be induced under specific conditions, allowing investigation of ciliary gene functions.
Mechanistically, ALMS1 localizes to centrosomes and basal bodies, scaffolding interactions with cytoskeletal and trafficking proteins. It binds ??-actinin and myosin to regulate actin dynamics, and interacts with IFT88 and BBSome proteins (e.g., BBS4) to facilitate ciliary assembly and protein transport. ALMS1 also modulates mTOR signaling by acting upstream of mTORC1, influencing Raptor and S6K. Cell cycle regulators and ciliary assembly signals control ALMS1 expression. Loss of ALMS1 disrupts these interactions, impairing ciliogenesis, mTOR signaling, and cellular stress responses.
In Jurkat cells, ALMS1 knockout enables dissection of centrosomal protein function in mTOR pathway regulation and T-cell biology. mTOR signaling governs T-cell activation, metabolism, and proliferation, and its dysregulation contributes to leukemogenesis. ALMS1 disruption in this leukemic background provides insights into how ciliopathy genes affect non-ciliated cells and offers a platform to study the interplay between centrosome-associated proteins and oncogenic signaling.
These knockout cells support applications in Alstr?m syndrome modeling, ciliopathy research, and mTOR signaling studies. Representative assays include western blotting for ALMS1 and phospho-S6K, immunofluorescence for ciliary markers, RT-qPCR, flow cytometry for cell cycle, and ciliogenesis assays. For more information, contact Ascent Research.
