The ALOX12 Knockout NCI-H1703 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population generated by targeted gene disruption of ALOX12 in the human NCI-H1703 cell line. This product provides a heterogeneous pool of edited cells harboring loss-of-function mutations at the ALOX12 locus, enabling functional studies without the constraints of monoclonal selection.
The parental NCI-H1703 cell line is derived from a patient with non-small cell lung cancer and exhibits features of squamous cell carcinoma, making it a relevant model for studying lung cancer biology. These cells display aggressive growth characteristics and are widely used to investigate signaling mechanisms, metastatic behavior, and therapeutic responses in lung carcinomas.
ALOX12 encodes arachidonate 12-lipoxygenase, which catalyzes the calcium- and iron-dependent oxygenation of arachidonic acid to 12(S)-hydroxyeicosatetraenoic acid (12-HETE). This lipid mediator acts through the GPR31 receptor to activate downstream MAPK cascades, including ERK1/2 and p38, as well as the PI3K/Akt pathway, thereby modulating cell proliferation and survival. ALOX12 expression is regulated by inflammatory cytokines such as TNF-?? and IL-1??, bacterial LPS, and growth factor EGF via transcription factors NF-??B and AP-1.
In the context of NCI-H1703 lung squamous carcinoma, ALOX12-derived 12-HETE signaling contributes to tumor cell proliferation, resistance to apoptosis, and enhanced migration. Disrupting ALOX12 in this model therefore offers a powerful approach to dissect the role of lipoxygenase-dependent lipid signaling in non-small cell lung cancer progression and to evaluate the therapeutic potential of targeting the arachidonic acid pathway, particularly in relation to drug sensitivity and metastatic spread.
These polyclonal knockout cells are applicable to a wide range of experimental assays, including lipidomic profiling of 12-HETE reduction, Western blot and RT-qPCR analysis of gene and protein expression changes, and functional studies using MTT proliferation assays, transwell migration/invasion tests, and Annexin V apoptosis detection. Phospho-protein analysis by Western blotting can monitor altered signaling through ERK, p38, and Akt. Additionally, they are suitable for drug sensitivity screens focusing on the lipoxygenase pathway. For further details or to discuss experimental design, please contact Ascent Research.
