The ANG Knockout A-549 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population with disruption of the angiogenin (ANG) gene in the A-549 human lung adenocarcinoma cell line. This pooled format provides a heterogeneous knockout culture, eliminating clonal bias and enabling robust functional genomics studies. The cells are generated using optimized CRISPR/Cas9 reagents for efficient target-gene disruption, serving as a versatile tool for angiogenin research.
The A-549 cell line is an adherent epithelial line derived from a lung adenocarcinoma of a 58-year-old Caucasian male. It is widely employed as a model for non-small cell lung cancer and respiratory epithelial biology, exhibiting properties such as alveolar type II differentiation and responsiveness to growth factors. These cells are tumorigenic in vivo and are instrumental in investigating oncogenic signaling, metastasis, and therapeutic resistance.
Angiogenin (ANG) is a secreted ribonuclease that promotes angiogenesis, cell proliferation, and rRNA transcription. Its expression is upregulated by hypoxia (via HIF-1??), VEGF, EGF, TNF-??, and IL-1??. Downstream, angiogenin activates the PI3K/Akt and ERK1/2 pathways, enhances eNOS activity, and stimulates rRNA synthesis via transcription factors UBF and RNA polymerase I. Interactions with extracellular matrix components like fibronectin and heparan sulfate proteoglycans facilitate endothelial cell migration and tube formation. In A-549 cells, ANG knockout disrupts these signals, attenuating proliferation, migration, and rRNA synthesis.
In the A-549 lung adenocarcinoma model, angiogenin loss provides a direct means to dissect tumor angiogenesis and angiogenin??s contribution to malignancy. These cells endogenously express angiogenin and are responsive to angiogenic cues, making the knockout system ideal for studying tumor?Cstromal crosstalk and hypoxia-driven signaling. Furthermore, because angiogenin deficiency is linked to amyotrophic lateral sclerosis (ALS) and vascular retinopathies, this model also enables exploration of neuroprotective and stress-response mechanisms.
Key applications include tube formation assays with conditioned medium, Western blotting for Akt and ERK phosphorylation, cell proliferation and migration assays, and rRNA synthesis analysis. RNA-seq can reveal global transcriptomic changes. The polyclonal knockout cells are also suitable for high-throughput screening of angiogenin-targeted therapeutics. For inquiries or to obtain this product, please contact Ascent Research.
