The ANKRD16 Knockout Jurkat Polyclonal Cells are a mixed population of Jurkat T lymphocytes engineered via CRISPR/Cas9 to disrupt the ANKRD16 gene, creating a loss-of-function model for studying ankyrin repeat domain-containing protein 16. This polyclonal knockout pool comprises cells with a range of gene-editing events, enabling bulk analysis of ANKRD16 deficiency without clonal selection. The product serves as a robust tool for functional genomics, protein interaction mapping, and immune cell biology investigations.
The Jurkat host cell line is an immortalized human T-lymphocyte line originally derived from a 14-year-old male with acute T-cell leukemia. Widely used as a model for T-cell receptor signaling, Jurkat cells recapitulate key aspects of T-cell activation, proliferation, and apoptosis. They provide a well-characterized system for probing signaling pathways relevant to immunology and leukemia biology, making them suitable for knockout studies aimed at uncovering novel regulators of T-cell function.
ANKRD16 encodes a protein containing ankyrin repeat motifs, which typically mediate protein-protein interactions. The protein is implicated in ciliary protein trafficking and photoreceptor homeostasis, with mutations leading to retinal dystrophies such as Leber congenital amaurosis and retinitis pigmentosa. ANKRD16 is reported to interact with IFT88, a core component of the intraflagellar transport complex essential for ciliary assembly and maintenance. Although Jurkat cells lack primary cilia, the knockout line provides a unique opportunity to explore cilia-independent roles of ANKRD16 in immune cell signaling and protein interaction networks.
In the context of Jurkat T cells, ANKRD16 deficiency may reveal novel functions beyond its established ciliary role. The polyclonal knockout population allows researchers to assess how loss of ANKRD16 affects T-cell receptor-mediated signaling, cytokine production, or proliferation, potentially uncovering adaptor-like functions mediated through ankyrin repeat domains. This model bridges the gap between ciliary biology and immunology, offering insights into the diverse functions of ankyrin repeat proteins in different cellular environments.
Typical applications include protein interaction studies via co-immunoprecipitation to confirm ANKRD16-IFT88 binding and identify new partners, functional analysis using flow cytometry for T-cell activation markers, and gene expression profiling by RT-qPCR. Western blotting and immunofluorescence enable validation of knockout efficiency and subcellular localization. Additionally, the polyclonal population is well-suited for CRISPR off-target analysis. This product accelerates research into ankyrin repeat protein biology and its implications in both ciliary and non-ciliary contexts. For further details, please contact Ascent Research.
