The ANKRD27 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting the ANKRD27 (VARP) gene in human HAP1 cells. This loss-of-function model enables study of ANKRD27-mediated processes without clonal isolation, minimizing clone-specific biases. The polyclonal format provides heterogeneous edits, ideal for bulk assays and genetic screens where population-level effects are informative.
HAP1 is a near-haploid human cell line derived from the KBM-7 chronic myeloid leukemia line. Its haploid karyotype in most cells simplifies gene targeting and phenotype interpretation, making it a workhorse for haploid genetic screens, CRISPR-based functional genomics, and drug?Ctarget interaction studies. The adherent morphology supports imaging assays, and the leukemic origin adds relevance to cancer research.
ANKRD27 (VARP) functions as a guanine nucleotide exchange factor for Rab21, promoting GTP loading and endosome-to-plasma membrane recycling of integrins, particularly ??5??1. It is recruited to endosomal membranes via direct binding to the retromer subunit VPS29 and collaborates with SNX27 to sort integrin cargo away from degradation. Key pathway components include Rab21, VPS29, VPS35, VPS26, SNX27, and integrin cytoplasmic tails. ANKRD27 thus acts downstream of retromer and upstream of integrin surface presentation, regulating adhesion and migration.
In HAP1 cells, ANKRD27 disruption yields a genetically clean model to dissect endocytic recycling without confounding second-allele effects. The knockout polyclonal population is suitable for quantitative assays of integrin trafficking, cell adhesion, and migration. Its haploid background also enhances the efficiency of genetic screens to identify modifiers of ANKRD27-dependent phenotypes, potentially revealing vulnerabilities in leukemia-related endosomal sorting.
Research applications include integrin recycling assays, cell migration and adhesion tests, co-immunoprecipitation of ANKRD27 complexes, and western blotting for Rab21 activation. The polyclonal cells are also well-suited for haploid genetic screens and retromer functional studies. For custom knockout generation or bulk orders, please contact Ascent Research.
