The ANKZF1 Knockout Jurkat Polyclonal Cells are a polyclonal population of Jurkat cells engineered via CRISPR/Cas9-mediated disruption of the ANKZF1 gene. This product provides a mixed pool of edited cells carrying diverse loss-of-function mutations, enabling robust functional studies without clonal bias. As a knockout model, it allows researchers to dissect ANKZF1’s role as a negative regulator of NF-??B signaling in a T cell context.
The Jurkat host cell line is an immortalized human T lymphocyte line derived from the peripheral blood of a 14-year-old male with acute T cell leukemia. Widely used as a model for T cell signaling and acute lymphoblastic leukemia, Jurkat cells exhibit constitutive activation of signaling pathways relevant to lymphocyte biology. Their rapid proliferation and well-characterized signaling networks make them an ideal platform for genetic manipulation and pathway analysis.
ANKZF1 encodes a protein containing ankyrin repeats and a zinc finger domain that functions as an inhibitor of the canonical NF-??B pathway. Mechanistically, ANKZF1 interacts with IKK-?? (NEMO) and the IKK complex to suppress I??B-?? phosphorylation, thereby preventing NF-??B p65/p50 nuclear translocation and transcriptional activation. Upstream signals such as TNF-?? and IL-1, acting through the TNF receptor, normally relieve this inhibition. In the knockout cells, disruption of ANKZF1 leads to enhanced IKK activity, increased I??B-?? degradation, and elevated NF-??B-dependent transcription of pro-inflammatory cytokines (e.g., IL-2, TNF-??) and anti-apoptotic genes.
In Jurkat T cells, NF-??B signaling is crucial for T cell activation, proliferation, and survival. ANKZF1 knockout in this background is expected to potentiate NF-??B responses, providing a unique tool to investigate how dysregulated NF-??B activity influences T cell biology and leukemogenesis. This model allows direct assessment of ANKZF1’s impact on T cell receptor-mediated signaling, cytokine production, and resistance to apoptosis, all of which are key processes in inflammation and T cell malignancies.
The ANKZF1 knockout polyclonal cells are well-suited for a range of research applications, including interrogation of NF-??B signaling regulation in T cells, functional genomics screens, and studies on leukemia cell survival and drug responses. Representative assays include Western blotting for phosphorylated p65 and I??B-??, NF-??B luciferase reporter assays, flow cytometry for apoptosis (Annexin V/PI), cytokine ELISA for IL-2 and TNF-??, cell viability assays (MTT), and quantitative PCR for NF-??B target genes. For further information or custom requests, please contact Ascent Research.
