The ANLN Knockout HAP1 Polyclonal Cells product constitutes a CRISPR/Cas9-edited polyclonal knockout cell population in which the ANLN gene has been disrupted within the HAP1 cell line. This loss-of-function model enables the study of ANLN-dependent biological processes without the need for single-cell clonal isolation, providing a heterogeneous population that retains near-haploid genetic simplicity.
The parental HAP1 cell line is a human near-haploid chronic myeloid leukemia (CML) line with an adherent, fibroblast-like morphology derived from the KBM-7 line. Its near-haploid karyotype, with only one copy of most chromosomes except for a portion of chromosome 15, facilitates efficient CRISPR-mediated knockout generation and minimizes complications from homologous recombination, making it a favored model for genetic screens and functional knockout studies.
ANLN encodes anillin, an actin-binding scaffold that organizes the contractile ring and midbody during cytokinesis. It is phosphorylated by CDK1 and acts downstream of RhoA signaling. Anillin directly binds active RhoA, F-actin, myosin II, and septins (SEPT2, SEPT6, SEPT7) to drive furrow ingression, while interactions with the RhoGEF ECT2 and kinase CIT-K regulate midbody maturation. Transcriptional control is mediated by FOXM1 and E2F1, and AKT modulates protein stability, integrating mitotic and growth signals at the division plane.
In the HAP1 near-haploid background, disruption of ANLN results in a powerful model to dissect cytokinesis defects. Because ANLN is essential for contractile ring assembly, its loss typically leads to multinucleation, failed abscission, or apoptosis??phenotypes readily observable in this simplified genomic context. This enables clean interpretation of genotype?Cphenotype relationships in studies of mitosis and cancer cell proliferation, where anillin overexpression has been implicated in breast, lung, and liver cancers.
This polyclonal knockout population is well-suited for applications including immunofluorescence microscopy to visualize cytokinetic structures, Western blotting for protein expression analysis, flow-cytometry-based DNA content profiling to assess ploidy abnormalities, and live-cell imaging to track division defects in real time. It also supports drug sensitivity assays for anti-mitotic agents and wound healing assays exploring anillin’s role in migration. For further technical information or custom options, please contact Ascent Research.
