The ANLN Knockout SK-HEP-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from the human hepatocellular carcinoma cell line SK-HEP-1. This product features targeted disruption of the ANLN gene using CRISPR/Cas9 technology, generating a heterogeneous pool of knockout cells. The polyclonal format avoids clonal artifacts and enables robust phenotypic screening of ANLN-dependent pathways without the need for single-cell cloning.
The host cell line, SK-HEP-1, is a human hepatocellular carcinoma cell line with mesenchymal characteristics, commonly used as a model for liver cancer metastasis and epithelial-mesenchymal transition (EMT). These cells exhibit a metastatic phenotype and provide a clinically relevant context for studying tumor cell migration, invasion, and the molecular mechanisms driving hepatocellular carcinoma progression.
ANLN encodes anillin, an actin-binding protein essential for cytokinesis, actomyosin contractility, and cell migration. Anillin scaffolds the contractile ring by interacting with RhoA, septins (SEPT2, SEPT6, SEPT7), actin, and myosin II, stabilizing cleavage furrow ingression. Its activity is regulated by upstream factors including transcription factors E2F1, FOXM1, MYC, and kinases CDK1 and Plk1, as well as RhoA GTPase. Downstream, anillin interacts with ECT2, Rac1, and PI(4,5)P2, positioning it as a central node in RhoA signaling and actin cytoskeleton organization.
In hepatocellular carcinoma, ANLN overexpression promotes proliferation, migration, and invasion, and correlates with poor prognosis. The ANLN knockout SK-HEP-1 polyclonal cells thus provide a valuable tool to dissect anillin??s role in liver cancer. Disruption of ANLN in this mesenchymal-like line is expected to impair cytokinesis, leading to binucleation and polyploidy, while reducing metastatic potential. This model enables investigation of ANLN-dependent EMT processes and RhoA-driven cytoskeletal dynamics.
Applications include Western blotting and RT-qPCR for ANLN validation, immunofluorescence for cleavage furrow defects, and flow cytometry for polyploidy assessment. Functional assays such as Transwell migration/invasion, MTT proliferation, and RhoA activation assays can be performed. These cells are ideal for studying cytokinesis failure, cancer cell motility, and ANLN-dependent drug sensitivities. For further details, please contact Ascent Research.
