This product is a CRISPR/Cas9-edited polyclonal knockout cell population targeting the ANXA10 gene in HEK293T cells. The polyclonal format provides a heterogeneous pool of gene-disrupted cells, generated through CRISPR/Cas9-mediated gene disruption without single-cell cloning. This loss-of-function model enables robust characterization of ANXA10-dependent cellular processes in a widely used human embryonic kidney epithelial background.
HEK293T cells are a derivative of the HEK293 line, stably expressing the SV40 large T antigen, which facilitates episomal replication of plasmids containing the SV40 origin of replication. This feature, combined with high transfectability, makes HEK293T a preferred host for viral production, recombinant protein expression, and diverse cell biology applications. The epithelial morphology and kidney origin provide a physiologically relevant context for studying membrane trafficking and signaling.
ANXA10 encodes a calcium-dependent phospholipid-binding protein belonging to the annexin family. It localizes to endosomal membranes and is implicated in endosomal trafficking and exocytosis, interacting with calcium ions, phospholipids, and S100 proteins. Mechanistically, ANXA10 functions downstream of intracellular calcium signals and is modulated by epigenetic silencing via promoter methylation, particularly in gastric and hepatocellular carcinomas. Representative pathway components include ANXA10, calcium ions, phospholipids, and Rab GTPases, linking ANXA10 to vesicular transport dynamics.
Disruption of ANXA10 in HEK293T cells is anticipated to impair calcium-dependent membrane association and perturb endosomal cargo sorting and exocytic release. This knockout model thus provides a powerful tool to dissect ANXA10’s role in endosome-mediated processes and its putative tumor suppressor activity, in a cell line amenable to genetic manipulation and high-resolution imaging. The polyclonal population offers a rapid means to assess gene function without clonal selection artifacts.
Researchers can employ these ANXA10 knockout HEK293T polyclonal cells to investigate endosomal trafficking using immunofluorescence with endosomal markers, quantify calcium flux responses, and measure regulated exocytosis. Knockout validation is readily performed via western blotting or RT-qPCR. The model is suitable for functional studies in calcium signaling, drug screening for compounds that modulate exocytosis, and tumor biology assays exploring ANXA10’s growth-suppressive functions. For further information, please contact Ascent Research.
