The ANXA4 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population with targeted gene disruption of ANXA4. This model provides a genetically heterogeneous loss-of-function system in Jurkat T lymphocytes for studying annexin A4 biology without clonal selection.
Jurkat cells, derived from a patient with acute T cell leukemia, are a widely used T lymphocyte model for T cell activation, signaling, and apoptosis. Their established signaling pathways and ease of genetic manipulation make them suitable for investigating immune cell function and leukemogenesis.
Annexin A4 is a calcium-dependent phospholipid-binding protein that regulates membrane repair, exocytosis, apoptosis, and ion channel function. In response to elevated intracellular calcium, ANXA4 translocates to membranes and interacts with phospholipids, S100 proteins (such as S100A6), actin, and other annexins like ANXA2. These interactions facilitate membrane resealing and modulate downstream signaling. ANXA4 is also linked to NF-??B pathway activation: it can influence the phosphorylation and activation of IKBKB, leading to nuclear translocation of NF-??B transcription factors RELA and NFKB1, which drive expression of anti-apoptotic and pro-survival genes. Additionally, ANXA4 participates in calcium flux regulation and affects the expression of cell adhesion molecules, integrating diverse cellular signals.
In Jurkat T cells, ANXA4 knockout disrupts calcium-dependent membrane repair and impairs NF-??B signaling, resulting in altered T cell activation, increased apoptosis, and decreased survival under stress conditions. This phenotype mirrors key aspects of T cell leukemia pathogenesis and provides a platform to investigate the role of ANXA4 in other malignancies such as colorectal, breast, and gastric cancers, where ANXA4 has been associated with tumor progression and epithelial-mesenchymal transition. By uncoupling calcium homeostasis from transcriptional responses, this model enables dissection of the signaling crosstalk that sustains lymphocyte viability.
These polyclonal knockout cells are ideal for T cell signaling, apoptosis, drug screening, and cancer biology studies. Representative assays include western blotting for ANXA4 and NF-??B targets, flow cytometry for activation and apoptosis markers, RT-qPCR for NF-??B targets, calcium flux measurements, NF-??B reporter assays, co-immunoprecipitation of ANXA4 interactors, and migration/invasion assays. For further technical specifications or to discuss how this model can support your research, please contact Ascent Research.
