The ANXA5 Knockout HeLa Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from HeLa cells, engineered to disrupt the ANXA5 gene. This product provides a heterogeneous pool of gene-edited cells, enabling loss-of-function studies in a well-characterized human epithelial cancer background. The polyclonal format offers robust representation of various knockout alleles, facilitating investigation of ANXA5 function without clonal selection bias.
HeLa cells, the host line, are a cervical adenocarcinoma cell line positive for human papillomavirus type 18 (HPV-18). These immortalized epithelial cells are widely used in biomedical research due to their stable growth and relevance to cancer biology. The HeLa background provides a context for studying tumor-associated processes such as proliferation, apoptosis, and membrane dynamics, making it an appropriate system for examining the roles of ANXA5 in oncogenic pathways.
ANXA5 encodes annexin A5, a calcium-dependent phospholipid-binding protein that preferentially associates with phosphatidylserine. Upon calcium elevation, ANXA5 binds to phosphatidylserine-exposing membranes, forming a lattice that inhibits coagulation and modulates apoptotic cell clearance. Upstream regulators include Ca2+, EGF, TNF-alpha, and glucocorticoids, while downstream effectors comprise phosphatidylserine, integrins, and F-actin. ANXA5 interacts directly with actin, integrin beta-5, and protein kinase C, participating in signaling networks that involve caspase activation and cytoskeletal rearrangement. This positions ANXA5 at the intersection of coagulation, apoptosis, and membrane repair pathways.
In the HeLa cervical adenocarcinoma context, ANXA5 disruption allows researchers to dissect its contribution to cancer cell behaviors such as apoptosis evasion, membrane repair, and interactions with the coagulation cascade. Given the HPV-18 positive status and cancerous phenotype, this knockout model may elucidate how calcium-mediated phosphatidylserine shielding influences immune evasion and metastatic potential. The model is particularly relevant for studying thrombotic disorders, autoimmune diseases, and cardiovascular diseases, where phosphatidylserine externalization and coagulation are dysregulated.
This polyclonal knockout product is suitable for a range of experimental applications, including apoptosis detection via Annexin V flow cytometry, cancer cell biology studies, membrane repair assays, and anticoagulation research. Key techniques include Western blotting for protein expression analysis, immunofluorescence for subcellular localization, calcium-dependent liposome binding assays to assess functional binding, and coagulation assays to evaluate procoagulant activity. For additional technical specifications or support, please contact Ascent Research.
