The ANXA6 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the ANXA6 gene in HeLa cells. This product offers a heterogeneous pool of gene-disrupted cells, providing a loss-of-function model for Annexin A6 studies without single-cell cloning. The polyclonal format enables population-level analyses of ANXA6-dependent functions while maintaining genetic diversity. The knockout population is supplied as a stable, ready-to-use culture, facilitating mechanistic and phenotypic investigations.
HeLa cells are an immortalized human cervical adenocarcinoma line, widely used in cancer research. They are HPV18-positive, with viral oncoproteins E6 and E7 inactivating p53 and Rb, respectively, thereby disrupting critical tumor suppressor pathways. This genetic background supports robust proliferation and allows stable genetic manipulation, making HeLa cells a standard platform for studying oncogenic signaling. The ANXA6 knockout in this context provides a relevant model to explore annexin functions in cervical cancer cell biology.
Annexin A6 is a calcium-dependent membrane-binding protein implicated in endocytosis, exocytosis, membrane repair, and signal transduction. It interacts with EGFR, F-actin, SNARE proteins, S100 proteins, and cholesterol, and its activity is regulated by EGF, calcium, PKC, and Src. ANXA6 modulates the Ras-MAPK pathway downstream of EGFR, influencing Ras, Raf, MEK, ERK1/2, and p38. Additionally, it impacts cholesterol homeostasis and actin cytoskeleton remodeling. Knockout of ANXA6 disrupts these interactions, altering EGFR trafficking and downstream signaling that govern cell migration and proliferation.
In HeLa cells, loss of ANXA6 is expected to perturb EGFR membrane dynamics and MAPK signal regulation. Given the reliance of these cells on EGFR-driven proliferation, ANXA6 knockout may alter migratory and proliferative behaviors. The compromised p53 and Rb checkpoints further sensitize the cells to mitogenic cues, making this knockout useful to dissect ANXA6??s role as a potential tumor modulator. Additionally, changes in cholesterol distribution may affect receptor tyrosine kinase signaling platforms, offering insights into membrane-related cancer mechanisms.
These polyclonal knockout cells are suitable for cancer biology, signal transduction, membrane trafficking, and drug resistance studies. Representative assays include Western blotting, RT-qPCR, immunofluorescence, flow cytometry, migration/invasion assays, co-immunoprecipitation, phospho-signaling analysis (e.g., phospho-ERK1/2), and apoptosis assays. The polyclonal format enables robust population-level readouts and is compatible with high-throughput screens. For detailed product information or technical support, please contact Ascent Research.
