The APC Knockout HAP1 Polyclonal Cells constitute a polyclonal population of HAP1 cells engineered by CRISPR/Cas9-mediated gene disruption of the adenomatous polyposis coli (APC) tumor suppressor gene. This heterogeneous knockout pool provides a loss-of-function model for investigating APC-dependent processes without clonal selection, maintaining genetic diversity that may better reflect population-level responses. The polyclonal format is ideal for pooled screening applications and studies where monoclonal artifacts are a concern.
HAP1 is a near-haploid human cell line originally derived from the KBM-7 chronic myelogenous leukemia (CML) cell line. Its near-haploid karyotype reduces genetic redundancy, facilitating unambiguous gene editing and phenotypic analysis. HAP1 cells display a fibroblast-like morphology and retain key signaling pathways relevant to CML research. The line is widely utilized as a robust model for studying kinase signaling, cell adhesion, and apoptosis, offering a consistent and reproducible platform for functional genomics.
APC is a critical tumor suppressor that serves as a scaffold in the ??-catenin destruction complex. It interacts with AXIN, GSK3??, and CK1?? to promote phosphorylation-dependent ubiquitination and proteasomal degradation of ??-catenin. Upon Wnt stimulation, Dishevelled inhibits the complex, stabilizing ??-catenin, which translocates to the nucleus and activates TCF/LEF transcription factors, inducing targets like MYC, CCND1, and AXIN2. APC also binds EB1 and microtubules, modulating cell migration, adhesion, and chromosomal stability.
In HAP1 cells, APC loss leads to constitutive ??-catenin stabilization and TCF/LEF-driven transcription, mimicking oncogenic mutations. The near-haploid background facilitates genetic interaction studies, allowing clear dissection of APC??s tumor suppressor functions and its role in cytoskeletal regulation. Combined with the CML origin, this model permits investigation of Wnt pathway cross-talk in leukemia biology.
These polyclonal knockout cells are suitable for Wnt signaling studies, cancer research, and drug screening. Typical assays include western blotting for ??-catenin, TOPFlash reporter, immunofluorescence, proliferation, colony formation, and migration assays. Drug sensitivity testing and RT-qPCR for Wnt targets further support mechanistic investigations. For technical inquiries, please contact Ascent Research.
