The APOE Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population featuring targeted disruption of the human APOE gene. This model provides a genetically defined loss-of-function system for investigating apolipoprotein E biology in a widely used human embryonic kidney cell background. The polyclonal nature ensures a heterogeneous knockout pool, suitable for experiments that do not require clonal homogeneity.
The HEK293T cell line is an immortalized human embryonic kidney epithelial cell line expressing the SV40 large T antigen, enabling high-efficiency episomal replication of plasmids containing the SV40 origin of replication. This host is renowned for robust transfectability, high-level recombinant protein expression, and broad utility in viral particle production, making it a preferred platform for gene editing and functional genomics studies.
APOE encodes apolipoprotein E, a pivotal lipid carrier that mediates cholesterol and phospholipid transport by binding to low-density lipoprotein receptor family members such as LRP1, LDLR, and VLDLR. Transcription is regulated by nuclear receptors LXR??, PPAR??, and RXR in response to cellular lipid status and inflammatory signals TNF?? and IL-1??. APOE functionality is isoform-dependent; the APOE4 variant disrupts amyloid-beta clearance and promotes aggregation, enhancing tau phosphorylation via receptor-mediated pathways. Downstream targets include lipid transporters ABCA1 and ABCG1, while APOE-LRP1 interactions influence NF-??B signaling and neuronal repair.
In the HEK293T context, APOE knockout enables dissection of receptor-mediated lipid transport mechanisms without interference from endogenous apolipoprotein E. This model is particularly useful for studying the impact of APOE loss on cholesterol efflux, A?? metabolism, and receptor recycling, as HEK293T cells express relevant receptors like LRP1 and can be co-transfected with variant constructs. The absence of neuronal lineage markers simplifies investigation of fundamental lipid handling and signaling pathways mediated by APOE.
Research applications include Alzheimer??s disease modeling through A?? uptake and clearance assays, cholesterol efflux measurements, and drug screening for APOE4-related pathologies. The cells are suitable for viral entry mechanism studies, protein interaction analyses via co-immunoprecipitation, and functional validation using Western blotting, ELISA, RT-qPCR, and immunofluorescence. This product supports high-content studies in lipid metabolism and neurodegenerative signaling. For further details or assistance with experimental design, please contact Ascent Research.
