ARL6IP5 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the ARL6IP5 gene in the human HAP1 cell line. This heterogeneous pool of cells with CRISPR/Cas9-mediated gene disruption provides a loss-of-function model for functional genomics and pathway analysis, eliminating the need for single-cell cloning and enabling cost-effective, reproducible studies of ARL6IP5-related processes.
HAP1 is a near-haploid human cell line derived from the KBM-7 chronic myelogenous leukemia line. Its haploid karyotype allows efficient CRISPR/Cas9-mediated knockout, as a single allele disruption results in functional loss. HAP1 is widely used for genetic screens and functional genomics, retaining key cancer-relevant signaling pathways.
ARL6IP5 encodes a protein that regulates glutamate transporter EAAC1 (SLC1A1) trafficking and actin cytoskeleton dynamics, influencing cell migration and stress responses. It interacts with ARL6, ??-actin, and ??-tubulin, and is regulated by p53 and oxidative stress. Downstream, ARL6IP5 modulates MAPK/ERK and PI3K/Akt pathways, with effects on MAPK1/3, AKT1, and BCL2, linking extracellular signals to cell survival and motility. Knockout of ARL6IP5 disrupts EAAC1 surface expression, impairs glutamate uptake, and leads to aberrant cytoskeletal reorganization and altered signaling, providing a model for excitotoxicity and migration studies.
In HAP1 cells, the ARL6IP5 knockout leverages the haploid background for high-penetrance phenotypic screening. This model is instrumental for dissecting mechanisms of cancer cell migration, drug resistance, and ER stress, as well as neuroprotective pathways relevant to ischemic injury and neurodegeneration. The hematopoietic origin also supports leukemia-related studies, while the polyclonal format facilitates robust, reproducible assays.
Researchers can use these cells in western blotting, RT-qPCR, and immunofluorescence to validate knockout and assess downstream molecules, glutamate uptake assays to measure EAAC1 function, wound healing assays to study migration, and flow cytometry for phospho-ERK/Akt analysis. Typical applications include glutamate transport studies, cancer cell migration research, neuroprotection mechanism investigations, drug resistance studies, and ER stress response analysis. For further information, please contact Ascent Research.
