The ARMC8 Knockout Jurkat Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal cell population derived from the Jurkat human T-lymphocyte line, engineered to disrupt the ARMC8 gene. This knockout model provides a heterogeneous pool of cells with targeted gene disruption, enabling robust loss-of-function studies within an immortalized T-cell leukemia background. The polyclonal format preserves genetic diversity while ensuring consistent ablation of ARMC8 function across the population, making it suitable for bulk biochemical and phenotypic analyses.
Jurkat cells are a human CD4+ T-cell leukemia line derived from acute lymphoblastic leukemia, widely used for studying T-cell signaling and apoptosis. They exhibit rapid growth and maintain key oncogenic pathways such as MAPK/ERK and PI3K/Akt, making them a robust model for cancer research. The immortalized nature of Jurkat cells facilitates genetic manipulation and long-term functional studies, providing an ideal host for dissecting genes like ARMC8.
ARMC8 is a constituent of the CTLH E3 ubiquitin ligase complex, where it interacts with MAEA, RMND5A, and WDR26 to stabilize ??-catenin (CTNNB1) and activate Akt/mTOR signaling. Upstream regulators include WNT3A, EGF, and TCF/LEF transcription factors, while miR-940 suppresses ARMC8 expression. Downstream, ARMC8 drives CTNNB1 accumulation and TCF7L2-mediated transcription of CCND1, and promotes phosphorylation of AKT1 and MTOR, thereby enhancing survival and proliferation. Crosstalk with the MAPK/ERK pathway further amplifies oncogenic output. Disruption of ARMC8 uncouples these networks, offering a precise tool for signaling studies.
In the Jurkat T-leukemia context, ARMC8 supports proliferation and apoptosis resistance via ??-catenin and Akt/mTOR. Knockout disrupts these pathways, impairing cell growth and increasing apoptotic susceptibility. This polyclonal model thus enables interrogation of CTLH complex roles in leukemogenesis and identification of ARMC8-dependent vulnerabilities.
This knockout cell population is suitable for Western blotting of CTNNB1 and phospho-AKT1, TOP/FOP luciferase reporter assays, flow cytometry for cell cycle analysis, and Annexin V apoptosis assays. Proliferation and tumorigenicity can be assessed by MTT/CCK-8 and colony formation assays, while co-immunoprecipitation probes CTLH complex assembly. Applications span cancer biology, T-cell leukemia research, and ubiquitin ligase characterization. For further information, contact Ascent Research.
