The ARMC9 Knockout SK-HEP-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population for loss-of-function studies of ARMC9. The polyclonal format minimizes clonal artifacts, ensuring a representative genetic background. Supplied as a ready-to-use pool, this knockout model is suited for diverse assays including signaling analysis, ciliary assessment, and drug screening in hepatic adenocarcinoma research.
The SK-HEP-1 host cell line was established from ascites of a liver adenocarcinoma patient and exhibits endothelial-like features alongside its epithelial origin, making it a valuable model for investigating tumor biology, angiogenesis, and epithelial-mesenchymal transitions. These cells are extensively utilized in hepatocellular carcinoma research for proliferation, migration, invasion, and drug response studies, and provide a robust background for gene editing.
ARMC9 is a ciliary protein critical for intraflagellar transport (IFT), interacting with IFT20, IFT88, and KIF3B, and integrating into IFT-A/B complexes and the BBSome. Its expression is regulated by RFX transcription factors and Notch signaling. ARMC9 modulates Wnt/??-catenin signaling downstream of Wnt3a and Frizzled, affecting ??-catenin stability. Knockout thus impairs ciliogenesis and attenuates Wnt pathway activity.
In SK-HEP-1 cells, ARMC9 disruption compromises primary cilium formation and intraflagellar transport, leading to altered Wnt/??-catenin signaling that may influence proliferation and differentiation. Given the established links between ciliary dysfunction and liver tumorigenesis, this model is highly relevant for dissecting hepatocellular carcinoma mechanisms and ciliopathies such as Joubert syndrome, particularly the role of ??-catenin dysregulation. It also serves as a platform for drug screening targeting ciliopathy-related hepatic cancers.
This polyclonal knockout pool supports multiple applications: Western blot and RT-qPCR for knockout confirmation; immunofluorescence with acetylated ??-tubulin or Arl13b to assess ciliary integrity; TOP/FOP flash reporter assays for Wnt pathway activity; MTT proliferation, migration, and invasion assays; and RNA-seq for transcriptomic profiling. It enables detailed mechanistic studies and drug screening targeting ciliary and Wnt signaling in hepatic adenocarcinoma. For further information, contact Ascent Research.
