The ARPP21 Knockout HeLa Polyclonal Cells product provides a heterogeneous population of HeLa cells with targeted disruption of the ARPP21 gene via CRISPR/Cas9-mediated gene editing. This polyclonal knockout pool serves as a robust loss-of-function model for studying ARPP21-dependent signaling pathways and cellular processes. The polyclonal format avoids clonal artifacts and represents the genetic heterogeneity of the knockout effect, making it ideal for screening applications and pathway analysis.
The host HeLa cell line is an immortalized cervical adenocarcinoma epithelial cell line derived from HPV18-transformed human cervical cancer tissue. HeLa is a widely used mammalian cell line valued for robust growth, ease of culture, and extensive molecular characterization. Its epithelial origin and transformed state render it suitable for signal transduction, cancer biology, and drug response studies. In the ARPP21 context, HeLa cells express cAMP/PKA and calcium signaling components, enabling functional interrogation in a relevant epithelial cancer setting.
ARPP21 is a cAMP-regulated calmodulin-binding inhibitor of protein phosphatase 1 (PP1). Upon PKA phosphorylation, ARPP21 binds calmodulin and inhibits PP1, modulating dephosphorylation of targets like CREB and actin-associated proteins. It functions downstream of the dopamine D1 receptor, Gs protein, adenylyl cyclase, and PKA, with activity also influenced by calcium/calmodulin pathways. Thus, ARPP21 integrates cAMP and calcium signals to regulate phosphorylation dynamics.
ARPP21 disruption in HeLa cells permits dissection of its contributions to cAMP-mediated signaling and cytoskeletal regulation in an epithelial cancer model. HeLa cells harbor active cAMP/PKA and calcium networks; ARPP21 knockout likely perturbs PP1-dependent dephosphorylation, altering actin organization, cell motility, or CREB-mediated gene expression. This model enables study of ARPP21-mediated phosphatase control in a cervical cancer background, informing pathways dysregulated in tumors with altered ARPP21 expression.
Researchers can apply this polyclonal knockout pool in assays such as Western blotting for ARPP21 phosphorylation, cAMP measurement, calcium flux analysis, co-immunoprecipitation with calmodulin, PP1 activity assays, and actin cytoskeleton immunofluorescence. These cells are also suited for high-throughput screening of small-molecule modulators targeting ARPP21 function or downstream components. For further technical details or product support, please contact Ascent Research.
