The ART1 Knockout HGC-27 Polyclonal Cells are a heterogeneous population of HGC-27 gastric carcinoma cells engineered by CRISPR/Cas9 to disrupt the ART1 gene. This polyclonal knockout pool allows loss-of-function studies of ART1 in a gastric cancer context. The model is designed to eliminate the mono-ADP-ribosyltransferase activity of ART1, enabling investigation of its role in integrin signaling and metastasis.
HGC-27 cells were isolated from a lymph node metastasis of a gastric adenocarcinoma patient and are widely used to study gastric cancer biology, including adhesion, migration, and invasion. The cell line retains epithelial characteristics and responds to inflammatory cytokines, making it an appropriate host for modeling ART1-dependent pathways.
ART1 is a GPI-anchored ecto-enzyme that catalyzes arginine-specific mono-ADP-ribosylation of extracellular proteins, primarily integrin ??1. Upstream regulators such as TNF-??, IL-6, and TGF-?? stimulate ART1 expression, leading to enhanced modification of integrin ??7??1 complexes. This promotes integrin clustering and activation of focal adhesion kinase (FAK), Src, and ERK1/2, which then drive expression of matrix metalloproteinases (MMPs) including MMP9. ART1 also interacts with defensin HNP-1 and ECM components to regulate cell adhesion. In the knockout cells, disruption of ART1 prevents this signaling cascade, impairing FAK phosphorylation and ERK1/2 activation.
In HGC-27 gastric cancer cells, ART1 knockout reduces integrin activation, cell adhesion, and migratory capacity. The loss of ART1 attenuates the FAK/Src/ERK pathway, which is critical for epithelial-mesenchymal transition and metastatic behavior. This model thus enables dissection of the molecular mechanisms linking integrin modification to gastric cancer progression and could be used to study interactions with the inflammatory tumor microenvironment.
The ART1 Knockout HGC-27 Polyclonal Cells are suitable for a range of assays including Western blot analysis of ART1 and downstream phospho-proteins, in vitro ADP-ribosylation activity measurements, Transwell migration and invasion assays, cell adhesion assays, and integrin activation profiling. They are applicable in functional genomics investigations, drug target validation, and inhibitor screening campaigns aimed at targeting ART1-mediated signaling in gastric cancer metastasis. For more details, please contact Ascent Research.
