ASGR1 Knockout 143B Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Bone
Disease:
Osteosarcoma

Datasheet

The ASGR1 Knockout 143B Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population of the ASGR1 gene in human 143B osteosarcoma cells. ASGR1 encodes the major subunit of the asialoglycoprotein receptor (ASGPR), which mediates clathrin-dependent endocytosis and lysosomal degradation of desialylated glycoproteins through interactions with ASGR2, clathrin, and the AP2 adaptor complex. This model is suited for studying ASGR1 function in osteosarcoma, glycosylation-dependent cancer mechanisms, viral entry, and hepatic clearance. Key applications include endocytosis assays using fluorescent asialoorosomucoid, western blot, and cell proliferation assays. The polyclonal format facilitates heterogeneity analysis.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

143B

Age

13 years

Gene Name

ASGR1

Gene Identifier

NCBI Gene ID 432

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

DMEM/F12

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The ASGR1 Knockout 143B Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population designed for targeted disruption of the human ASGR1 gene.

This model provides a heterogeneous loss-of-function resource in the 143B osteosarcoma background, enabling pooled functional studies without clonal isolation.

The polyclonal format preserves the diversity of editing outcomes, offering a system to investigate gene function in a population-relevant context.