The ASGR1 Knockout Ca Ski Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the ASGR1 gene in the human cervical carcinoma cell line Ca Ski. This product provides a robust loss-of-function model to investigate the role of the asialoglycoprotein receptor 1 (ASGR1) in endocytosis, glycoprotein metabolism, and signaling. As a polyclonal knockout pool, cells harbor heterogeneous disruptions enabling pooled functional studies.
Ca Ski is a human cervical carcinoma epithelial line derived from a metastatic squamous cell carcinoma, containing integrated HPV16 DNA with constitutive E6/E7 expression. It is a widely used model for cervical cancer research, including viral oncoprotein function, epithelial carcinogenesis, and HPV-driven pathways. The epithelial origin and malignant phenotype make it a relevant host for studying genes influencing tumor cell behavior, adhesion, and immune modulation.
ASGR1 encodes the major subunit of the asialoglycoprotein receptor, which forms hetero-oligomers with ASGR2 to mediate clathrin-dependent endocytosis of desialylated glycoproteins via interactions with adaptor AP2 and clathrin. In non-hepatic cells, ASGR1 may engage JAK2/STAT3 signaling downstream of IL-6 and upstream regulators HNF4A, CEBPA, and STAT3, influencing NFKB1 and lysosomal enzyme expression. This positions ASGR1 at the intersection of receptor-mediated endocytosis and proliferative/survival pathways.
The functional role of ASGR1 in cervical cancer is not well established, making this knockout model valuable. Its depletion may affect cell adhesion and immune-related interactions, especially given ASGR1’s links to NF-??B and JAK-STAT pathways frequently altered in HPV-driven cancers. This model enables investigation of cross-talk between HPV oncoproteins and the host endocytic machinery, potentially revealing new targets.
Key applications include western blotting, RT-qPCR, immunofluorescence, and flow cytometry for confirming ASGR1 disruption and surface expression. Researchers can perform endocytosis assays with fluorescent asialoorosomucoid, proliferation, apoptosis, and migration assays, and co-immunoprecipitation for ASGR1?CASGR2 interactions. This product supports studies in glycobiology, cancer biology, and drug targeting. For further information, contact Ascent Research.
