The B3GAT3 Knockout HeLa Polyclonal Cells product offers a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the B3GAT3 gene within the HeLa cervical adenocarcinoma line. This polyclonal pool provides a loss-of-function model without requiring single-cell cloning, enabling efficient investigation of ??-1,3-glucuronyltransferase 3 function in a well-established human epithelial system.
HeLa cells are an immortalized human cervical cancer line widely employed for studies in cancer biology, signal transduction, and extracellular matrix research. Their rapid proliferation, robust culture characteristics, and extensive literature support make them a reliable host for genetic perturbation and subsequent phenotypic analysis.
B3GAT3 encodes ??-1,3-glucuronyltransferase 3, a Golgi-resident enzyme that catalyzes the transfer of glucuronic acid to elongating heparan sulfate and chondroitin sulfate chains, an essential step in proteoglycan biosynthesis. Its expression is transcriptionally regulated by SOX9, TGFB1, and FGF2. B3GAT3 functions in concert with interacting glycosyltransferases EXT1, EXT2, and CHSY1, along with CHPF, to assemble mature glycosaminoglycan structures. Disruption of B3GAT3 impairs the synthesis of downstream heparan sulfate and chondroitin sulfate proteoglycans, thereby affecting growth factor signaling pathways (FGF, Wnt, Hedgehog) and compromising extracellular matrix integrity.
In the HeLa context, loss of B3GAT3 activity alters the cell surface proteoglycan repertoire and modulates responses to exogenous growth factors, making this model valuable for dissecting matrix-dependent signaling and cell adhesion. It serves as a relevant system for studying B3GAT3-related connective tissue disorders, including linkeropathies characterized by multiple joint dislocations, short stature, and craniofacial dysmorphism.
Research applications encompass glycosaminoglycan biology, proteoglycan function, and extracellular matrix research. Typical assays include Western blotting and RT-qPCR to confirm knockout, immunofluorescence for localization, heparan sulfate and chondroitin sulfate ELISAs for quantification, proteoglycan electrophoresis, wound healing assays to assess migration, and mass spectrometry for glycan profiling. This product supports connective tissue disease modeling and growth factor signaling modulation. For further details or technical inquiries, please contact Ascent Research.
