The B4GALT7 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population for disruption of the B4GALT7 gene in the HAP1 near-haploid human cell line. This polyclonal loss-of-function model enables investigation of B4GALT7??s role in proteoglycan biosynthesis without clonal selection, providing a heterogeneous pool of gene-edited cells suitable for population-level assays and pooled screening.
The HAP1 cell line, derived from KBM-7 chronic myeloid leukemia cells, features a near-haploid karyotype that streamlines genetic manipulation and ensures high-efficiency knockout generation. Widely used in CRISPR functional genomics, HAP1 reduces genetic redundancy and allows for clear phenotype interpretation, making it ideal for studying pathways related to extracellular matrix biology and cancer.
B4GALT7 catalyzes galactose transfer to xylose on proteoglycan core proteins, initiating glycosaminoglycan (GAG) chain assembly. Its activity is regulated by SOX9 and TGF-beta signaling. B4GALT7 cooperates with B3GAT3, B3GALT6, EXTL3, EXT1, and EXT2 to build the tetrasaccharide linker and extend GAG chains onto core proteins such as aggrecan and decorin. This enzymatic step is critical for proper proteoglycan modification and extracellular matrix organization. Loss of B4GALT7 function disrupts GAG biosynthesis and alters interactions within glycosyltransferase complexes, impairing downstream signaling and structural integrity.
In the HAP1 near-haploid background, B4GALT7 knockout provides a simplified system to examine the consequences of defective GAG initiation. The polyclonal population exhibits a range of editing outcomes, enabling robust detection of impaired sulfated GAG production via DMMB assay and altered proteoglycan expression by western blotting. This model is particularly suitable for studying cell adhesion, migration, and signal transduction, with sharp phenotypes due to reduced genetic redundancy. It also allows investigation of interactions with TGF-beta and SOX9 pathways in connective tissue disorder contexts.
These polyclonal knockout cells support diverse applications including proteoglycan function analysis, GAG biosynthesis studies, Ehlers-Danlos syndrome modeling, and extracellular matrix research. Typical assays include DMMB quantification, western blotting for aggrecan and decorin, immunofluorescence of ECM components, RT-qPCR for target genes, and cell migration experiments. For further details, customization, or control products, please contact Ascent Research.
