The BASP1 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population engineered to eliminate functional expression of the BASP1 gene in the HAP1 human cell line. This loss-of-function model enables robust investigation of BASP1-dependent mechanisms through targeted gene disruption, providing a heterogeneous pool of edited cells suitable for bulk functional studies without clonal isolation.
HAP1 is a near-haploid human cell line derived from the KBM-7 chronic myeloid leukemia (CML) line, originating from a male patient in blast crisis. Its hematopoietic origin and near-haploid karyotype simplify genetic analysis by reducing gene copy number, making it an ideal host for knockout studies. HAP1 retains key features of CML, serving as a relevant model for leukemia biology while offering advantages in genetic screening and signaling pathway dissection.
BASP1 is a membrane-associated protein that integrates extracellular signals to regulate neurite outgrowth, synaptic plasticity, and gene transcription. It is a prominent substrate of protein kinase C (PKC), phosphorylated in response to neuronal activity and growth factors. BASP1 binds calmodulin in a calcium-dependent manner and interacts with actin cytoskeletal components, linking signaling to morphological changes. Additionally, BASP1 functions as a transcriptional co-suppressor by directly binding the Wilms?? tumor protein WT1 to repress WT1 target gene expression. Representative pathway components include GAP-43, PKC, calmodulin, and actin, highlighting BASP1??s dual role in cytoskeletal dynamics and transcriptional regulation.
Expressing BASP1 in a hematopoietic model extends its functional analysis beyond neural contexts. In HAP1 cells, BASP1 may modulate PI3K/AKT signaling and WT1-mediated transcription, processes implicated in leukemic cell growth and differentiation. Knockout of BASP1 thus provides a platform to investigate its contributions to CML blast crisis biology, including effects on cell proliferation, migration, and stress responses, while also enabling cross-tissue comparisons of BASP1-dependent pathways.
This polyclonal knockout product supports a wide range of applications. In cancer research, the cells can be used in migration and invasion assays, complemented by western blotting for phospho-signaling analysis of PI3K/AKT. Transcriptional regulation studies benefit from RT-qPCR and reporter assays to quantify WT1-target expression. The population??s heterogeneity facilitates genetic screening, and immunofluorescence can resolve actin cytoskeletal alterations. Neurological applications include assessing neurite outgrowth under differentiation conditions. For additional information or custom orders, please contact Ascent Research.
