BATF3 Knockout CaSki Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Uterus (cervix)
Disease:
Squamous cell carcinoma

Datasheet

This product is a CRISPR/Cas9-edited polyclonal BATF3 knockout population generated from the Ca Ski human cervical carcinoma cell line, an HPV16-positive epithelial model. BATF3 is a critical transcription factor in the IRF8-BATF3 network, controlling genes such as IL12B and TAP1 that are essential for antigen cross-presentation and antiviral immunity. Disruption of BATF3 in this tumor context enables dissection of immune evasion programs and antigen processing in cervical cancer. Applications include tumor-immune coculture assays, cytokine response studies, and genome-wide transcriptomic analyses to explore BATF3-dependent immune signaling. This polyclonal knockout resource supports investigation of HPV-driven oncogenic mechanisms and screening for modulators of tumor immunogenicity in a clinically relevant cellular background.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

CaSki

Sex of Donor

Female

Age

40 years

Derived From Site

Metastatic; Small intestine

Gene Name

BATF3

Gene Identifier

NCBI Gene ID 55509

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The BATF3 Knockout Ca Ski Polyclonal Cells product consists of a heterogeneous population of Ca Ski cells edited via CRISPR/Cas9 to disrupt the BATF3 gene, yielding a polyclonal knockout model.

This loss-of-function system enables robust interrogation of BATF3-dependent molecular mechanisms without clonal isolation, preserving the complexity of the parental line while abolishing target-gene function across the pool.

Researchers receive a ready-to-use polyclonal knockout cell population, suitable for direct application in downstream assays requiring an isogenic background with uniform genetic disruption.