The BATF3 Knockout NCI-H1299 Polyclonal Cells are a population of NCI-H1299 lung adenocarcinoma cells with CRISPR/Cas9-mediated disruption of the BATF3 gene. As a polyclonal pool, this product provides a heterogeneous loss-of-function model that reflects the diverse editing outcomes typical of genome editing, enabling robust assessment of BATF3-dependent phenotypes without clonal bias.
The NCI-H1299 parental line is an epithelial, p53-null non-small cell lung carcinoma cell line derived from a lymph node metastasis. Its aggressive growth characteristics and metastatic origin make it a standard model for studying lung cancer biology, including proliferation, migration, and immune evasion mechanisms, in a genetic background devoid of p53 checkpoint activity.
BATF3 is an AP-1 transcription factor that heterodimerizes with JUN and cooperatively interacts with IRF4 and IRF8 to regulate gene expression networks. It is activated downstream of FLT3L and Toll-like receptor signaling via STAT1/STAT3, and functions in concert with PU.1 and IRF8 to drive the development of conventional type 1 dendritic cells and antigen cross-presentation. Key transcriptional targets include ID2, ZBTB46, XCR1, CLEC9A, and the cytokines IL-12b, CXCL9, and CXCL10, which collectively mediate CD8+ T cell priming and anti-tumor immunity.
In NCI-H1299 cells, BATF3 knockout eliminates this transcriptional circuitry, offering a system to dissect its tumor-intrinsic roles. This model is particularly suited to investigate BATF3-dependent cytokine production, surface molecule expression, and responses to interferon or TLR signals, all of which impact tumor-immune interactions. The p53-null environment allows focused interrogation of AP-1-driven pathways without interference from p53-mediated transcriptional programs.
This polyclonal knockout population is suitable for a variety of molecular and functional assays, including Western blotting, RT-qPCR, and RNA-seq to confirm gene disruption and assess transcriptomic changes, as well as ChIP-qPCR for promoter occupancy studies. Functional studies can include proliferation, Transwell migration, colony formation, and apoptosis assays, alongside drug sensitivity testing and co-culture setups to model immune cell interactions. For further information, please contact Ascent Research.
