BICC1 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HEK293T cell line. These cells carry a targeted disruption of the BICC1 gene, providing a loss-of-function model to study the role of this RNA-binding protein in cell signaling, development, and disease. The polyclonal format ensures a heterogeneous population of edited cells, suitable for bulk functional assays without clonal selection bias.
HEK293T cells are a widely used derivative of the HEK293 cell line, originating from human embryonic kidney tissue. They stably express the SV40 large T antigen, which enables episomal replication of plasmids containing the SV40 origin of replication, leading to high transfectability and robust protein expression. These adherent cells are female in origin and are extensively employed in viral production, transient overexpression, and CRISPR-based genome editing studies. Their epithelial morphology and rapid growth make them an ideal platform for investigating signaling pathways and protein function.
BICC1 (BicC family RNA-binding protein 1) encodes an RNA-binding protein that post-transcriptionally regulates mRNA translation and stability. It is a critical modulator of the Wnt/??-catenin and planar cell polarity (PCP) pathways. BICC1 interacts with molecular partners including ANKS3, ANKS6, Dvl2, and Prickle1 to control the subcellular localization and translation of key Wnt components. Mechanistically, BICC1 binds to the 3?? UTRs of targets such as Cripto-1, Cyclin D1, c?Myc, and AXIN2, thereby influencing ???catenin/TCF/LEF?mediated transcription. Upstream, BICC1 is regulated by Wnt ligands (e.g., Wnt3a) and Dishevelled (Dvl). Disruption of BICC1 perturbs the balance of the Wnt signaling network, affecting processes from embryonic axis determination to kidney development.
In the HEK293T context, BICC1 knockout allows dissection of its role in a human epithelial cell line with active Wnt signaling. The loss of BICC1 may alter the expression of downstream targets like Cyclin D1 and c-Myc, impacting cell proliferation, migration, and differentiation. Because HEK293T cells are highly transfectable, this knockout model is particularly suited for rescue experiments with mutant or wild?type BICC1 constructs, luciferase reporter assays for Wnt activity, and co?transfection studies with pathway components such as Dvl2 or ???catenin. The polyclonal nature of the knockout mimics a population-level gene disruption, which can be advantageous for studying overall pathway responses without the artifacts of clonal variability.
Typical applications include investigating BICC1 function in Wnt signaling, cell migration, and colony formation, as well as small-molecule screening. Assays such as Western blotting, RT-qPCR, immunofluorescence for ??-catenin, and dual-luciferase reporters are commonly used. This model is relevant to congenital heart defects, renal cysts, and cancer. For further information, please contact Ascent Research.
