The BLOC1S3 Knockout HT29 Polyclonal Cells product offers a CRISPR/Cas9-edited polyclonal knockout cell population for functional studies of the BLOC1S3 gene. Derived from HT29 human colorectal adenocarcinoma cells, this heterogeneous pool provides a robust loss-of-function model without clonal selection biases, suitable for investigating BLOC1S3-dependent processes in an epithelial context.
HT29 cells, isolated from a female patient, serve as a well-established intestinal epithelial model. These adherent cells recapitulate enterocyte-like polarity and are widely used in colorectal cancer research to study tumorigenesis, migration, and drug responses, making them an appropriate host for probing endosomal trafficking pathways relevant to both normal and malignant intestinal biology.
BLOC1S3 encodes BLOS3, a core subunit of the BLOC-1 complex essential for biogenesis of lysosome-related organelles. BLOC-1 functions downstream of the retromer VPS26/VPS35/SNX6 and small GTPases Rab32/Rab38, regulated by ANKRD27/Varp, and interacts with AP-3, BLOC-2, and SNARE proteins syntaxin 13 and VAMP7. Disruption of BLOC1S3 impairs trafficking of melanogenic enzymes TYR, TYRP1, DCT, the copper transporter ATP7A, and lysosomal membrane proteins LAMP1 and CD63 to specialized organelles, while general endocytosis remains largely unaffected.
In HT29 colorectal cancer cells, BLOC1S3 knockout provides a unique model to dissect how defects in lysosome-related organelle biogenesis intersect with epithelial tumor biology. Because mutations in BLOC1S3 cause Hermansky-Pudlak syndrome type 8, this polyclonal knockout tool enables investigation of the epithelial manifestations of this disorder and the potential contribution of trafficking defects to colorectal cancer cell proliferation, migration, and invasion.
Typical applications include western blotting and RT-qPCR for knockout validation, immunofluorescence for mislocalized LAMP1 and TYR, flow cytometry for CD63 surface expression, and functional assays such as transwell migration and MTT viability. The cells also support ultrastructural analysis by electron microscopy and studies of BLOC-1 complex function in tumorigenesis and endosomal sorting in intestinal epithelia. For technical inquiries, contact Ascent Research.
