The BRPF3 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed for functional studies of the BRPF3 gene. This product provides a heterogeneous pool of HAP1 cells harboring gene disruptions at the BRPF3 locus, enabling researchers to investigate loss-of-function phenotypes without the need for clonal selection. The polyclonal format ensures robust representation of knockout alleles, making it suitable for pooled screening and bulk analysis in biomedical research.
HAP1 is a near-haploid human cell line originally derived from the KBM-7 chronic myelogenous leukemia (CML) cell line. Its haploid karyotype simplifies genetic manipulation and ensures efficient knockout generation, as a single mutation can ablate gene function. HAP1 cells retain key cellular pathways and are widely employed for CRISPR-based knockout studies, providing a genetically tractable model for functional genomics, high-throughput screening, and the study of cell-autonomous gene functions.
BRPF3 encodes a scaffold protein that assembles the HBO1 histone acetyltransferase complex, which includes the catalytic subunit HBO1 (KAT7), ING5, and EAF6. This complex acetylates histones H3 at lysines 14 and 23 (H3K14ac, H3K23ac) and histone H4 at lysines 5 and 12 (H4K5ac, H4K12ac), thereby promoting chromatin relaxation and transcriptional activation. BRPF3-mediated acetylation is critical for DNA replication licensing by facilitating origin firing. Upstream, BRPF3 is regulated by developmental transcription factors and cell cycle signals, while downstream it modulates target gene expression and replication origin activity. The complex directly interacts with histones H3 and H4 and utilizes acetyl-CoA as a cofactor.
In HAP1 cells, knockout of BRPF3 disrupts the HBO1 complex, creating a clean loss-of-function model to study histone acetylation dynamics and DNA replication. The haploid background allows efficient mutagenesis and homogeneous phenotypic outcomes, which is advantageous for quantitative assays and drug response profiling. This model is particularly relevant for exploring BRPF3-associated neurodevelopmental disorders and potential cancer predisposition, as the unperturbed control HAP1 cells exhibit robust growth and are amenable to pharmacological perturbations.
This knockout product can be employed in diverse experimental workflows, including Western blotting for histone acetylation marks (e.g., H3K14ac, H4K5ac), ChIP-qPCR to assess BRPF3 target occupancy, RNA-seq for transcriptome-wide changes, and flow cytometry for cell cycle distribution analysis. It is well-suited for functional genomics screens targeting epigenetic regulators, drug sensitivity profiling with HDAC inhibitors, and mechanistic studies of replication licensing. For additional product details and lot-specific performance data, please contact Ascent Research.
