The BTN1A1 Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the A2780 human ovarian carcinoma epithelial cell line, designed for loss-of-function studies of the BTN1A1 gene. This polyclonal pool provides a genetically heterogeneous model to interrogate BTN1A1 function without clonal selection bias, suitable for pooled screening and functional assays.
The parental A2780 cell line was established from an untreated patient with ovarian endometrioid carcinoma, serving as a well-characterized model for ovarian cancer research. These adherent epithelial cells retain key oncogenic signaling pathways and metabolic features relevant to ovarian tumor biology.
BTN1A1 encodes butyrophilin, an immunoglobulin superfamily member critically involved in milk fat globule secretion through complex formation with xanthine oxidoreductase (XDH) and perilipin-2 (PLIN2), facilitating lipid droplet envelopment and release. In ovarian cancer cells, BTN1A1 may additionally influence immune cell interactions via structural homology to B7 co-stimulatory molecules. Upstream, BTN1A1 expression is regulated by prolactin, STAT5, and inflammatory cytokines such as TNF-alpha. Downstream, it impacts lipid droplet secretion and potentially modulates T cell activity through interactions with T cell receptors and apolipoproteins.
In the A2780 ovarian cancer context, disruption of BTN1A1 allows dissection of its dual roles in lipid metabolism and immune regulation. Loss of BTN1A1 may alter lipid droplet dynamics, impacting metabolic flux and tumor cell viability, while simultaneously compromising potential immune checkpoint functions that modulate the tumor microenvironment. This knockout model thus enables investigation of BTN1A1’s contribution to ovarian cancer progression and immune evasion.
Typical applications include elucidating BTN1A1’s role in lipid metabolism via BODIPY staining and metabolic flux analysis, assessing impacts on cell proliferation, migration, and apoptosis, and dissecting immune modulatory functions through T cell co-culture and activation assays. Transcriptomic profiling by RNA-seq and targeted validation by RT-qPCR and Western blotting further support mechanistic studies. For further information or to discuss custom projects, please contact Ascent Research.
