The BV-2 cell line is a murine microglia cell line derived from C57BL/6 mice, transformed using a v-raf/v-myc carrying retrovirus (J2). BV-2 cells resemble primary microglia in both phenotypical and functional properties.
BV-2 express key surface and intracellular markers typical of primary microglia, including Iba1, CD11b, F4/80, Toll-like receptor 4 (TLR4). The expression level of these markers changes with the immune response of BV-2 to inflammatory stimuli, such as LPS and IFN-γ. After stimulation, BV-2 cells also produce cytokines, like TNF-α, IL-1β, IL-6, and generate nitric oxide (NO) and reactive oxygen species (ROS). During in vitro culture, BV-2 cells grow in resting state. exhibiting small cell bodies with ramified (branched) processes. Upon stimulation, they retract processes and become amoeboid, rounder, and more adherent.
The bacterial, yeast, and fungi are not detected in these cells by daily monitor.
Mycoplasma testing
Negative for mycoplasma through PCR analysis
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Intended Use
This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.
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